PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 91
Page iii
... polymerase chain reaction ( PCR ) in 1985 , the number of different applications has grown steadily , as have the modifications of the basic method . This increase has been claimed to be exponential - like the PCR itself . In any case ...
... polymerase chain reaction ( PCR ) in 1985 , the number of different applications has grown steadily , as have the modifications of the basic method . This increase has been claimed to be exponential - like the PCR itself . In any case ...
Page x
... Polymerase Chain Reaction 105 Howard Ochman , James W. Ajioka , Dan Garza , and Daniel L. Hartl 11 . 12 . Alu PCR : The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources 113 David L. Nelson and C. Thomas ...
... Polymerase Chain Reaction 105 Howard Ochman , James W. Ajioka , Dan Garza , and Daniel L. Hartl 11 . 12 . Alu PCR : The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources 113 David L. Nelson and C. Thomas ...
Page 1
... polymerase chain reaction ( PCR ) , represents such a change . The PCR is an in vitro method for the enzymatic synthesis of specific DNA sequences , using two oligonucleotide primers that hybridize to opposite strands and flank the ...
... polymerase chain reaction ( PCR ) , represents such a change . The PCR is an in vitro method for the enzymatic synthesis of specific DNA sequences , using two oligonucleotide primers that hybridize to opposite strands and flank the ...
Page 2
... reaction which could now be automated by a thermal cycling device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried ...
... reaction which could now be automated by a thermal cycling device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried ...
Page 3
... reaction . This plateau is reached later ( e.g. , about 30 cycles rather than 20 starting with 1 μg of genomic DNA ) in the Taq PCR than in the reaction with the Klenow enzyme due to the increased specificity of the former reactions ...
... reaction . This plateau is reached later ( e.g. , about 30 cycles rather than 20 starting with 1 μg of genomic DNA ) in the Taq PCR than in the reaction with the Klenow enzyme due to the increased specificity of the former reactions ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube