PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page iii
... protocols , listing favorite " PCR recipes , " while others give an overview of a particular field . My hope is that an interested reader , armed with the knowledge of some of the methodological issues and of some of the applications ...
... protocols , listing favorite " PCR recipes , " while others give an overview of a particular field . My hope is that an interested reader , armed with the knowledge of some of the methodological issues and of some of the applications ...
Page 4
... protocol ( reduced Mg ** and dNTP concentrations ) , found a lower frequency of errors . " The original estimate is ... protocols for sample preparation on the PCR is discussed in Chapter 4 . The ability to amplify specific sequences ...
... protocol ( reduced Mg ** and dNTP concentrations ) , found a lower frequency of errors . " The original estimate is ... protocols for sample preparation on the PCR is discussed in Chapter 4 . The ability to amplify specific sequences ...
Page 11
... protocols include 10 % dimethyl sulfoxide ( DMSO ) ostensibly to reduce the secondary structure of the target DNA ; our own experience has shown that DMSO can be slightly inhibitory to Taq polymerase and decrease the overall yield of ...
... protocols include 10 % dimethyl sulfoxide ( DMSO ) ostensibly to reduce the secondary structure of the target DNA ; our own experience has shown that DMSO can be slightly inhibitory to Taq polymerase and decrease the overall yield of ...
Page 19
... protocol for native Taq DNA polymerase ( DHG , in preparation ) was in preparation ) was intended to yield a single polypeptide chain enzyme , we could have failed to detect an E. coli Pol III " ε - like " associated subunit . Taq DNA ...
... protocol for native Taq DNA polymerase ( DHG , in preparation ) was in preparation ) was intended to yield a single polypeptide chain enzyme , we could have failed to detect an E. coli Pol III " ε - like " associated subunit . Taq DNA ...
Page 31
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube