PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page iii
... Saiki , Stephen Scharf , Glenn Horn , Fred Faloona , Kary Mullis , and Norm Arnheim ) who first developed it for the analysis of human genetic variation . To David Gelfand and Susanne Stoffel who isolated and characterized the ...
... Saiki , Stephen Scharf , Glenn Horn , Fred Faloona , Kary Mullis , and Norm Arnheim ) who first developed it for the analysis of human genetic variation . To David Gelfand and Susanne Stoffel who isolated and characterized the ...
Page vii
... , Ph.D. Bioscience Specialist Perkin - Elmer Cetus Randall Saiki Associate Scientist Department of Human Genetics Cetus Corporation George Sensabaugh , Ph.D. Professor and Chairman Department of Public Contributors vii.
... , Ph.D. Bioscience Specialist Perkin - Elmer Cetus Randall Saiki Associate Scientist Department of Human Genetics Cetus Corporation George Sensabaugh , Ph.D. Professor and Chairman Department of Public Contributors vii.
Page ix
... Saiki 2. Taq DNA Polymerase 17 David Gelfand 3. PCR Automation 23 Christian Oste 4. Simple and Rapid Preparation of Samples for PCR 31 Russell Higuchi PART TWO RESEARCH APPLICATIONS 39 5 . Direct Sequencing of In Vitro Amplified DNA Ulf ...
... Saiki 2. Taq DNA Polymerase 17 David Gelfand 3. PCR Automation 23 Christian Oste 4. Simple and Rapid Preparation of Samples for PCR 31 Russell Higuchi PART TWO RESEARCH APPLICATIONS 39 5 . Direct Sequencing of In Vitro Amplified DNA Ulf ...
Page 5
... Saiki , R.K. , Horn , G.T. , and Erlich , H.A. ( 1986 ) Cold Spring Harbor Symp . Quant . Biol . 51 : 263-273 . 3. Saiki , R. , Scharf , S. , Faloona , F. , Mullis , K. , Horn , G. , Erlich , H.A. , and Arnheim , N. ( 1985 ) Science 230 ...
... Saiki , R.K. , Horn , G.T. , and Erlich , H.A. ( 1986 ) Cold Spring Harbor Symp . Quant . Biol . 51 : 263-273 . 3. Saiki , R. , Scharf , S. , Faloona , F. , Mullis , K. , Horn , G. , Erlich , H.A. , and Arnheim , N. ( 1985 ) Science 230 ...
Page 7
... Saiki INTRODUCTION In the few years since its introduction , 123 the polymerase chain reaction has already become a widespread research technique . Like the PCR itself , the numbers of its practitioners have been accumulating ...
... Saiki INTRODUCTION In the few years since its introduction , 123 the polymerase chain reaction has already become a widespread research technique . Like the PCR itself , the numbers of its practitioners have been accumulating ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube