PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 65
Page 1
... products synthesized in one cycle can serve as a template in the next , the number of target DNA copies approximately doubles at every cycle . Thus , 20 cycles of PCR yields about a million - fold ( 220 ) amplification . method , which ...
... products synthesized in one cycle can serve as a template in the next , the number of target DNA copies approximately doubles at every cycle . Thus , 20 cycles of PCR yields about a million - fold ( 220 ) amplification . method , which ...
Page 2
... device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the temperature within the reaction ...
... device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the temperature within the reaction ...
Page 3
... amplification products . The increase in the specificity of the Taq PCR results in an improved yield of the amplified target fragment by reducing the competition by non - target products for enzyme and primers . In the later cycles ...
... amplification products . The increase in the specificity of the Taq PCR results in an improved yield of the amplified target fragment by reducing the competition by non - target products for enzyme and primers . In the later cycles ...
Page 4
... amplification reaction is an issue with broad implications for both research and diagnostic applications . Given the capacity of PCR to synthesize millions of DNA copies , contamination of the sample reaction with either products of a ...
... amplification reaction is an issue with broad implications for both research and diagnostic applications . Given the capacity of PCR to synthesize millions of DNA copies , contamination of the sample reaction with either products of a ...
Page 7
Principles and Applications for DNA Amplification Henry Erlich. CHAPTER 1 The ... amplification is achieved . In fact , the PCR is a relatively complicated ... products obtained . Although the results will be good in most cases , there ...
Principles and Applications for DNA Amplification Henry Erlich. CHAPTER 1 The ... amplification is achieved . In fact , the PCR is a relatively complicated ... products obtained . Although the results will be good in most cases , there ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube