PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 18
... assay , 2.0 mM magnesium chloride maximally stimulates Taq polymerase activity at 0.7-0.8 mM total dNTP . Higher concentrations of Mg ++ are inhibitory , with 40-50 % inhibition at 10 mM MgCl2 . Since deoxynucleotide triphosphates can ...
... assay , 2.0 mM magnesium chloride maximally stimulates Taq polymerase activity at 0.7-0.8 mM total dNTP . Higher concentrations of Mg ++ are inhibitory , with 40-50 % inhibition at 10 mM MgCl2 . Since deoxynucleotide triphosphates can ...
Page 19
... assay inhibits DNA synthesis by 50 % . While several investigators have observed that inclusion of 10 % DMSO facilitates certain PCR assays , it is not clear which parameters of PCR are affected . The presence of DMSO may affect the Tm ...
... assay inhibits DNA synthesis by 50 % . While several investigators have observed that inclusion of 10 % DMSO facilitates certain PCR assays , it is not clear which parameters of PCR are affected . The presence of DMSO may affect the Tm ...
Page 20
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Page 62
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube