PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 25
Page iii
... automated procedure and transformed the reaction from a method of last resort to one of first choice . To Corey Levenson , Dragan Spasic , and Lauri Goda who responded gracefully to the increased demand for synthetic oligonucleotides ...
... automated procedure and transformed the reaction from a method of last resort to one of first choice . To Corey Levenson , Dragan Spasic , and Lauri Goda who responded gracefully to the increased demand for synthetic oligonucleotides ...
Page iv
... automated thermocylers . Finally , this book would not have been possible without the patience and dedication of Kathy Levenson who worked closely with the authors and , with the help of Dean Grantham , transformed the sporadic trickle ...
... automated thermocylers . Finally , this book would not have been possible without the patience and dedication of Kathy Levenson who worked closely with the authors and , with the help of Dean Grantham , transformed the sporadic trickle ...
Page ix
... Automation 23 Christian Oste 4. Simple and Rapid Preparation of Samples for PCR 31 Russell Higuchi PART TWO RESEARCH APPLICATIONS 39 5 . Direct Sequencing of In Vitro Amplified DNA Ulf Gyllensten 45 6. Using PCR to Engineer DNA 61 ...
... Automation 23 Christian Oste 4. Simple and Rapid Preparation of Samples for PCR 31 Russell Higuchi PART TWO RESEARCH APPLICATIONS 39 5 . Direct Sequencing of In Vitro Amplified DNA Ulf Gyllensten 45 6. Using PCR to Engineer DNA 61 ...
Page 2
... automated by a thermal cycling device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the ...
... automated by a thermal cycling device ( see Chapter 3 ) . The reaction components ( template , primers , Taq polymerase , dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the ...
Page 5
... automated reaction . In its early , heady days , Erwin Chargaff once described molecular biology dismissively as " the practice of biochemistry without a license . " Its spectacular success over the last 30 years suggests that ...
... automated reaction . In its early , heady days , Erwin Chargaff once described molecular biology dismissively as " the practice of biochemistry without a license . " Its spectacular success over the last 30 years suggests that ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube