PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 2
... concentration as well as the temperature cycling profile ) on the specificity and yield of the amplification is discussed in Chapter 1. Although , for any given pair of oligonucleotide primers , an optimal set of conditions can be ...
... concentration as well as the temperature cycling profile ) on the specificity and yield of the amplification is discussed in Chapter 1. Although , for any given pair of oligonucleotide primers , an optimal set of conditions can be ...
Page 3
... concentration may also contribute to the plateau effect and are discussed in Chapter 1. In addition to the increase in the specificity and yield of PCR made possible by Taq polymerase , the use of this enzyme allows the amplification of ...
... concentration may also contribute to the plateau effect and are discussed in Chapter 1. In addition to the increase in the specificity and yield of PCR made possible by Taq polymerase , the use of this enzyme allows the amplification of ...
Page 4
... concentrations ) , found a lower frequency of errors . " The original estimate is in reasonable agreement with the report of a 10 nt error rate determined by the in vitro replication of a ẞ - galactosidase template by the Taq polymerase ...
... concentrations ) , found a lower frequency of errors . " The original estimate is in reasonable agreement with the report of a 10 nt error rate determined by the in vitro replication of a ẞ - galactosidase template by the Taq polymerase ...
Page 10
... concentrations ranging from 0.05 to 0.5 μM of each oligonucleotide should be acceptable . " Primer dimer " is an ... concentration of MgCl2 can have a profound effect on the specificity and yield of an amplification . Concentrations ...
... concentrations ranging from 0.05 to 0.5 μM of each oligonucleotide should be acceptable . " Primer dimer " is an ... concentration of MgCl2 can have a profound effect on the specificity and yield of an amplification . Concentrations ...
Page 11
... concentration of 0.8 mM ; this leaves 0.7 mM of the original 1.5 mM MgCl2 not complexed with dNTP . Consequently , if the dNTP concentration is changed significantly , a compensatory change in MgCl , may be necessary . Taq polymerase is ...
... concentration of 0.8 mM ; this leaves 0.7 mM of the original 1.5 mM MgCl2 not complexed with dNTP . Consequently , if the dNTP concentration is changed significantly , a compensatory change in MgCl , may be necessary . Taq polymerase is ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube