PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 69
Page 3
... ~ 1 / 400 and the error rate * After the first few cycles , virtually all of the templates have been synthesized in previous cycles and , therefore , contain the primer sequences . 10 was calculated to be ~ 2x10 nt / cycle Part One 3.
... ~ 1 / 400 and the error rate * After the first few cycles , virtually all of the templates have been synthesized in previous cycles and , therefore , contain the primer sequences . 10 was calculated to be ~ 2x10 nt / cycle Part One 3.
Page 4
... contain measurable 3 ' - to - 5 ' exonuclease " proof - reading " activity . An important property of the PCR , particularly in diagnostic applications , is the capacity to amplify a target sequence from crude DNA preparations * as well ...
... contain measurable 3 ' - to - 5 ' exonuclease " proof - reading " activity . An important property of the PCR , particularly in diagnostic applications , is the capacity to amplify a target sequence from crude DNA preparations * as well ...
Page 9
... containing standard buffer , 200 μM each dNTP , and 250 nM each primer , 100 ng human genomic DNA , and 2.5 units Taq polymerase ( Perkin Elmer / Cetus ) were subjected to 30 cycles of amplification . Two μl of each sample were resolved ...
... containing standard buffer , 200 μM each dNTP , and 250 nM each primer , 100 ng human genomic DNA , and 2.5 units Taq polymerase ( Perkin Elmer / Cetus ) were subjected to 30 cycles of amplification . Two μl of each sample were resolved ...
Page 10
... containing very few initial copies of template . It is a double- stranded fragment whose length is very close to the sum of the two primers and appears to occur when one primer is extended by the polymerase over the other primer . The ...
... containing very few initial copies of template . It is a double- stranded fragment whose length is very close to the sum of the two primers and appears to occur when one primer is extended by the polymerase over the other primer . The ...
Page 15
... containing 2.5 units of Taq polymerase , substrate excess conditions begin to occur around 1 μg of DNA ( 3 nmol of deoxynucleotide monophosphate ) . It can be overcome by increasing the extension time and / or increasing the amount of ...
... containing 2.5 units of Taq polymerase , substrate excess conditions begin to occur around 1 μg of DNA ( 3 nmol of deoxynucleotide monophosphate ) . It can be overcome by increasing the extension time and / or increasing the amount of ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube