PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 57
Page 1
... cycle can serve as a template in the next , the number of target DNA copies approximately doubles at every cycle . Thus , 20 cycles of PCR yields about a million - fold ( 220 ) amplification . method , which was invented by Kary Mullis ...
... cycle can serve as a template in the next , the number of target DNA copies approximately doubles at every cycle . Thus , 20 cycles of PCR yields about a million - fold ( 220 ) amplification . method , which was invented by Kary Mullis ...
Page 2
... cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the thermostable DNA polymerase ( Taq polymerase ) isolated from Thermus aquaticus ( see Chapter 2 ) transformed the PCR into a simple and ...
... cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the thermostable DNA polymerase ( Taq polymerase ) isolated from Thermus aquaticus ( see Chapter 2 ) transformed the PCR into a simple and ...
Page 3
... cycles , the amount of enzyme is no longer sufficient to extend all the annealed primer / template complexes in a single cycle period , resulting in a reduced efficiency and a " plateau " in the amplification reaction . This plateau is ...
... cycles , the amount of enzyme is no longer sufficient to extend all the annealed primer / template complexes in a single cycle period , resulting in a reduced efficiency and a " plateau " in the amplification reaction . This plateau is ...
Page 4
... cycle . More recent studies , carried out on a different gene using the current , optimized protocol ( reduced Mg ** and ... cycles required for analysis also minimizes the chance that a rare contaminating template will be amplified . A ...
... cycle . More recent studies , carried out on a different gene using the current , optimized protocol ( reduced Mg ** and ... cycles required for analysis also minimizes the chance that a rare contaminating template will be amplified . A ...
Page 8
... Cycle " program set to denature at 94 ° C for 20 sec , anneal at 55 ° C for 20 sec , and extend at 72 ° C for 30 sec for a total of 30 cycles . ( The " Step - Cycle " program causes the instrument to heat and cool to the target ...
... Cycle " program set to denature at 94 ° C for 20 sec , anneal at 55 ° C for 20 sec , and extend at 72 ° C for 30 sec for a total of 30 cycles . ( The " Step - Cycle " program causes the instrument to heat and cool to the target ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube