PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 29
Page 2
... dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the temperature within the reaction tube . The effect of varying the reaction parameters ( e.g. , enzyme , primer and Mg + ...
... dNTP's , and buffer ) could all be assembled and the amplification reaction carried out by simply cycling the temperature within the reaction tube . The effect of varying the reaction parameters ( e.g. , enzyme , primer and Mg + ...
Page 4
... dNTP concentrations ) , found a lower frequency of errors . " The original estimate is in reasonable agreement with the report of a 10 nt error rate determined by the in vitro replication of a ẞ - galactosidase template by the Taq ...
... dNTP concentrations ) , found a lower frequency of errors . " The original estimate is in reasonable agreement with the report of a 10 nt error rate determined by the in vitro replication of a ẞ - galactosidase template by the Taq ...
Page 9
... dNTP , and 250 nM each primer , 100 ng human genomic DNA , and 2.5 units Taq polymerase ( Perkin Elmer / Cetus ) were subjected to 30 cycles of amplification . Two μl of each sample were resolved on a 1.6 % agarose gel and visualized by ...
... dNTP , and 250 nM each primer , 100 ng human genomic DNA , and 2.5 units Taq polymerase ( Perkin Elmer / Cetus ) were subjected to 30 cycles of amplification . Two μl of each sample were resolved on a 1.6 % agarose gel and visualized by ...
Page 11
... dNTP ) , but in some circumstances , different amounts of Mg2 + may prove to be necessary ( Figure 2 ) . Generally , excess Mg2 + will result in the accumulation of non - specific amplification products and insufficient Mg2 + will ...
... dNTP ) , but in some circumstances , different amounts of Mg2 + may prove to be necessary ( Figure 2 ) . Generally , excess Mg2 + will result in the accumulation of non - specific amplification products and insufficient Mg2 + will ...
Page 14
... dNTP or inactivation of polymerase or dNTP , ( none of which is significant in a standard reaction ) , there remains three more exotic causes of plateau substrate excess conditions , competition by non - specific products 14 Design and ...
... dNTP or inactivation of polymerase or dNTP , ( none of which is significant in a standard reaction ) , there remains three more exotic causes of plateau substrate excess conditions , competition by non - specific products 14 Design and ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube