PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 49
Page ix
... Denaturing Gradient Gel Electrophoresis 71 Richard M. Myers , Val C. Sheffield , and David R. Cox 8 . Detection of Gene Expression 89 Ernest S. Kawasaki and Alice M. Wang 9 . PCR Amplification of Specific Sequences from a CDNA ix.
... Denaturing Gradient Gel Electrophoresis 71 Richard M. Myers , Val C. Sheffield , and David R. Cox 8 . Detection of Gene Expression 89 Ernest S. Kawasaki and Alice M. Wang 9 . PCR Amplification of Specific Sequences from a CDNA ix.
Page 1
... denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in the exponential accumulation of a specific fragment whose termini are defined by the 5 ' ends of the primers . Because the primer ...
... denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in the exponential accumulation of a specific fragment whose termini are defined by the 5 ' ends of the primers . Because the primer ...
Page 8
... denature at 94 ° C for 20 sec , anneal at 55 ° C for 20 sec , and extend at 72 ° C for 30 sec for a total of 30 cycles . ( The " Step - Cycle " program causes the instrument to heat and cool to the target temperatures as quickly as ...
... denature at 94 ° C for 20 sec , anneal at 55 ° C for 20 sec , and extend at 72 ° C for 30 sec for a total of 30 cycles . ( The " Step - Cycle " program causes the instrument to heat and cool to the target temperatures as quickly as ...
Page 11
... denaturation , annealing , and extension . This cycling can be accomplished either manually with pre - set water baths , or automatically with the DNA Thermal Cycler . In a typical reaction , the double - stranded DNA is denatured by ...
... denaturation , annealing , and extension . This cycling can be accomplished either manually with pre - set water baths , or automatically with the DNA Thermal Cycler . In a typical reaction , the double - stranded DNA is denatured by ...
Page 13
... denaturation . ) The ramp time , or time taken to change from one temperature to another , depends on the type of equipment used . With some notable exceptions , this rate of temperature change is not important and the fastest ramps ...
... denaturation . ) The ramp time , or time taken to change from one temperature to another , depends on the type of equipment used . With some notable exceptions , this rate of temperature change is not important and the fastest ramps ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube