PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 2
Principles and Applications for DNA Amplification Henry Erlich. human B - globin DNA and to the prenatal diagnosis of ... genomic template . " This effect was revealed by experiments in which normal genomic DNA ( with two copies of the B ...
Principles and Applications for DNA Amplification Henry Erlich. human B - globin DNA and to the prenatal diagnosis of ... genomic template . " This effect was revealed by experiments in which normal genomic DNA ( with two copies of the B ...
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... genomic DNA ) in the Taq PCR than in the reaction with the Klenow enzyme due to the increased specificity of the former reactions . Other factors like the reassociation of the template strands at high product concentration may also ...
... genomic DNA ) in the Taq PCR than in the reaction with the Klenow enzyme due to the increased specificity of the former reactions . Other factors like the reassociation of the template strands at high product concentration may also ...
Page 4
... DNA polymerases with an active " proof - reading " function , like T4 DNA ... genomic sequences ( see Chapter 19 ) all require rigorous measures to ... DNA is necessary to detect potential contamination . In genetic typing , a sample that ...
... DNA polymerases with an active " proof - reading " function , like T4 DNA ... genomic sequences ( see Chapter 19 ) all require rigorous measures to ... DNA is necessary to detect potential contamination . In genetic typing , a sample that ...
Page 8
... DNA sample will be variable , of course , but it will usually have between 102 to 105 copies of template ( e.g. , 0.1 μg human genomic DNA ) . A few drops of mineral oil are often added to seal the reaction and prevent condensation ...
... DNA sample will be variable , of course , but it will usually have between 102 to 105 copies of template ( e.g. , 0.1 μg human genomic DNA ) . A few drops of mineral oil are often added to seal the reaction and prevent condensation ...
Page 9
... genomic DNA , and 2.5 units Taq polymerase ( Perkin Elmer / Cetus ) were subjected to 30 cycles of amplification . Two μl of each sample were resolved on a 1.6 % agarose gel and visualized by ethidium bromide fluorescence . The lengths ...
... genomic DNA , and 2.5 units Taq polymerase ( Perkin Elmer / Cetus ) were subjected to 30 cycles of amplification . Two μl of each sample were resolved on a 1.6 % agarose gel and visualized by ethidium bromide fluorescence . The lengths ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube