PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Results 1-5 of 43
Page 1
... hybridize to opposite strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in ...
... hybridize to opposite strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in ...
Page 2
... subsequent analysis with a specific hybridization probe34 or , in some cases , with internal " nested primers " 1 to detect and characterize the amplified target sequence . The use of the Taq polymerase not only simplified the. 2 Part One.
... subsequent analysis with a specific hybridization probe34 or , in some cases , with internal " nested primers " 1 to detect and characterize the amplified target sequence . The use of the Taq polymerase not only simplified the. 2 Part One.
Page 4
... hybridization or in direct sequencing , the rare errors in individual products are not detectable . However , in the sequence analysis of individual clones derived from a PCR , sequences must be determined from multiple clones to ...
... hybridization or in direct sequencing , the rare errors in individual products are not detectable . However , in the sequence analysis of individual clones derived from a PCR , sequences must be determined from multiple clones to ...
Page 14
... hybridization occurs almost instantaneously and long incubation at the annealing temperature is not required . ( The 20 sec denaturation and annealing incubation times used with the Step - Cycle program on the Thermal Cycler is the ...
... hybridization occurs almost instantaneously and long incubation at the annealing temperature is not required . ( The 20 sec denaturation and annealing incubation times used with the Step - Cycle program on the Thermal Cycler is the ...
Page 40
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube