PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 3
... nucleotide substitutions , insertions , and deletions can also be introduced into the amplified product with the appropriate primers . Unlike these directed mutations , changes introduced into the sequence of the PCR products due to ...
... nucleotide substitutions , insertions , and deletions can also be introduced into the amplified product with the appropriate primers . Unlike these directed mutations , changes introduced into the sequence of the PCR products due to ...
Page 4
... nucleotides from the faithful copes of the template sequence . Other DNA polymerases with an active " proof - reading " function , like T4 DNA polymerase , can carry out PCR11 and may prove useful in studies where a very low error rate ...
... nucleotides from the faithful copes of the template sequence . Other DNA polymerases with an active " proof - reading " function , like T4 DNA polymerase , can carry out PCR11 and may prove useful in studies where a very low error rate ...
Page 18
... nucleotides / sec / enzyme molecule . Innis et al.1 have reported highly processive synthesis properties and an extension rate of > 60 nt / sec at 70 ° C with Taq DNA ... nucleotide triphosphates to yield 2.6 μg of DNA 18 Taq DNA Polymerase.
... nucleotides / sec / enzyme molecule . Innis et al.1 have reported highly processive synthesis properties and an extension rate of > 60 nt / sec at 70 ° C with Taq DNA ... nucleotide triphosphates to yield 2.6 μg of DNA 18 Taq DNA Polymerase.
Page 19
... nucleotide incorporation / misincorporation , biochemical fidelity measurements have indicated that the ability of " non - proofreading " DNA polymerases to misincorporate a deoxynucleotide triphosphate is determined critically by the ...
... nucleotide incorporation / misincorporation , biochemical fidelity measurements have indicated that the ability of " non - proofreading " DNA polymerases to misincorporate a deoxynucleotide triphosphate is determined critically by the ...
Page 39
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube