PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 30
Page 1
... oligonucleotide primers that hybridize to opposite strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers ...
... oligonucleotide primers that hybridize to opposite strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers ...
Page 2
... primers . This enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset ... oligonucleotide primers , an optimal set of conditions can be established , there is no single set of conditions ...
... primers . This enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset ... oligonucleotide primers , an optimal set of conditions can be established , there is no single set of conditions ...
Page 3
... primers that were mismatched with the template . At 37 ° C , many of these mismatched primers are sufficiently ... oligonucleotide primers become physically incorporated into the amplified product and mismatches between the 5 ' end of ...
... primers that were mismatched with the template . At 37 ° C , many of these mismatched primers are sufficiently ... oligonucleotide primers become physically incorporated into the amplified product and mismatches between the 5 ' end of ...
Page 7
... oligonucleotide primers , deoxynucleotide triphosphates , and the thermostable Taq DNA polymerase in a suitable buffer , then repetitively heating and cooling the mixture for several hours until the desired amount of amplification is ...
... oligonucleotide primers , deoxynucleotide triphosphates , and the thermostable Taq DNA polymerase in a suitable buffer , then repetitively heating and cooling the mixture for several hours until the desired amount of amplification is ...
Page 10
... primers . These guidelines only increase the chances that any given pair of oligonucleotides function properly , they are not absolute requirements . Most primers will be between 20 and 30 bases in length and the optimal amount to use ...
... primers . These guidelines only increase the chances that any given pair of oligonucleotides function properly , they are not absolute requirements . Most primers will be between 20 and 30 bases in length and the optimal amount to use ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube