PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 88
Page 1
... primers that hybridize to opposite strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA ...
... primers that hybridize to opposite strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA ...
Page 2
... primers . This enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset of each PCR cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the ...
... primers . This enzyme was inactivated by the high temperature required to separate the two DNA strands at the outset of each PCR cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the ...
Page 3
... primer annealing and extension , thereby increasing the overall stringency of the reaction and minimizing the extension of primers that were mismatched with the template . At 37 ° C , many of these mismatched primers are sufficiently ...
... primer annealing and extension , thereby increasing the overall stringency of the reaction and minimizing the extension of primers that were mismatched with the template . At 37 ° C , many of these mismatched primers are sufficiently ...
Page 7
... primers , deoxynucleotide triphosphates , and the thermostable Taq DNA polymerase in a suitable buffer , then repetitively heating and cooling the mixture for several hours until the desired amount of amplification is achieved . In fact ...
... primers , deoxynucleotide triphosphates , and the thermostable Taq DNA polymerase in a suitable buffer , then repetitively heating and cooling the mixture for several hours until the desired amount of amplification is achieved . In fact ...
Page 8
... PRIMER SELECTION Unfortunately , the approach to the selection of efficient and specific primers remains somewhat empirical . There is no set of rules that will ensure the synthesis of an effective primer pair . Yet it is the primers ...
... PRIMER SELECTION Unfortunately , the approach to the selection of efficient and specific primers remains somewhat empirical . There is no set of rules that will ensure the synthesis of an effective primer pair . Yet it is the primers ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube