PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
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Page 1
... region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in the exponential accumulation of a specific ...
... region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in the exponential accumulation of a specific ...
Page 12
... ) . Although similar in size and from the same region of the B - globin gene , the two primer pairs have very different magnesium optima . II II نه نه 3.0 4.0 6.0 8.0 10.0 8.0 4.0 825 0.25 Figure 3. Effect of Taq polymerase. 122.
... ) . Although similar in size and from the same region of the B - globin gene , the two primer pairs have very different magnesium optima . II II نه نه 3.0 4.0 6.0 8.0 10.0 8.0 4.0 825 0.25 Figure 3. Effect of Taq polymerase. 122.
Page 18
... regions of local intramolecular secondary structure on the template strand or a change in the ratio of the forward rate constant to the dissociation constant . Very little DNA synthesis is seen at very high temperatures ( > 90 ° C ...
... regions of local intramolecular secondary structure on the template strand or a change in the ratio of the forward rate constant to the dissociation constant . Very little DNA synthesis is seen at very high temperatures ( > 90 ° C ...
Page 20
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Page 21
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube