PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 38
Page ii
... Synthesis . 3. DNA polymerases - Biotechnology . I. Erlich , Henry A. , 1943- . II . Series . [ DNLM : 1. DNA Polymerases . 2. Gene Amplification . 3. Genetic Engineering . 4. Molecular Biology . 5. RNA Polymerases . QH 442 P348 ] QP606 ...
... Synthesis . 3. DNA polymerases - Biotechnology . I. Erlich , Henry A. , 1943- . II . Series . [ DNLM : 1. DNA Polymerases . 2. Gene Amplification . 3. Genetic Engineering . 4. Molecular Biology . 5. RNA Polymerases . QH 442 P348 ] QP606 ...
Page 1
... synthesis of specific DNA sequences , using two oligonucleotide primers that hybridize to opposite strands and flank ... synthesized in one cycle can serve as a template in the next , the number of target DNA copies approximately doubles ...
... synthesis of specific DNA sequences , using two oligonucleotide primers that hybridize to opposite strands and flank ... synthesized in one cycle can serve as a template in the next , the number of target DNA copies approximately doubles ...
Page 2
... synthesized , as expected ; however , several non - target fragments were produced . Thus , in the absence of the " right ... synthesis by the Klenow enzyme at 37 ° C was not highly specific . Although a specific target fragment could be ...
... synthesized , as expected ; however , several non - target fragments were produced . Thus , in the absence of the " right ... synthesis by the Klenow enzyme at 37 ° C was not highly specific . Although a specific target fragment could be ...
Page 3
... synthesis of RNA copies from the PCR product using T7 RNA polymerase . Furthermore , specific nucleotide ... synthesized in previous cycles and , therefore , contain the primer sequences . 10 was calculated to be ~ 2x10 nt / cycle Part One 3.
... synthesis of RNA copies from the PCR product using T7 RNA polymerase . Furthermore , specific nucleotide ... synthesized in previous cycles and , therefore , contain the primer sequences . 10 was calculated to be ~ 2x10 nt / cycle Part One 3.
Page 4
... synthesize millions of DNA copies , contamination of the sample reaction with either products of a previous reaction ( product carryover ) or with material from an exogenous source is a potential problem - particularly in those ...
... synthesize millions of DNA copies , contamination of the sample reaction with either products of a previous reaction ( product carryover ) or with material from an exogenous source is a potential problem - particularly in those ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube