PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 36
Page 2
... target sequence in the genomic template . " This effect was revealed by experiments in which normal genomic DNA ... target fragments were produced . Thus , in the absence of the " right " template , the " wrong " sequences became ...
... target sequence in the genomic template . " This effect was revealed by experiments in which normal genomic DNA ... target fragments were produced . Thus , in the absence of the " right " template , the " wrong " sequences became ...
Page 3
... target fragment by reducing the competition by non - target products for enzyme and primers . In the later cycles ... sequence , it is also a very powerful and precise way of altering a particular template sequence . Since the ...
... target fragment by reducing the competition by non - target products for enzyme and primers . In the later cycles ... sequence , it is also a very powerful and precise way of altering a particular template sequence . Since the ...
Page 4
... sequence analysis of individual clones derived from a PCR , sequences must be determined from multiple clones to ... target sequence from crude DNA preparations * as well as from degraded DNA templates.12 The DNA in a sample need not be ...
... sequence analysis of individual clones derived from a PCR , sequences must be determined from multiple clones to ... target sequence from crude DNA preparations * as well as from degraded DNA templates.12 The DNA in a sample need not be ...
Page 10
... sequences become incorporated into the double - stranded PCR product and provide a means of introducing restriction sites or regulatory elements ( e.g. , promoters ) at the ends of the amplified target sequence . " If required , shorter ...
... sequences become incorporated into the double - stranded PCR product and provide a means of introducing restriction sites or regulatory elements ( e.g. , promoters ) at the ends of the amplified target sequence . " If required , shorter ...
Page 11
... target DNA ; our own experience has shown that DMSO can be slightly ... sequence complexity , such as genomic DNA , there is an optimum ... target fragment ( Figure 3 ) . CYCLING PARAMETERS PCR is performed by incubating the samples at ...
... target DNA ; our own experience has shown that DMSO can be slightly ... sequence complexity , such as genomic DNA , there is an optimum ... target fragment ( Figure 3 ) . CYCLING PARAMETERS PCR is performed by incubating the samples at ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube