PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Results 1-5 of 56
Page 2
... temperature within the reaction tube . The effect of varying the reaction parameters ( e.g. , enzyme , primer and Mg + concentration as well as the temperature cycling profile ) on the specificity and yield of the amplification is ...
... temperature within the reaction tube . The effect of varying the reaction parameters ( e.g. , enzyme , primer and Mg + concentration as well as the temperature cycling profile ) on the specificity and yield of the amplification is ...
Page 3
... temperature optimum for the Taq polymerase ( ~ 75 ° C ) allowed the use of higher temperatures for primer annealing and extension , thereby increasing the overall stringency of the reaction and minimizing the extension of primers that ...
... temperature optimum for the Taq polymerase ( ~ 75 ° C ) allowed the use of higher temperatures for primer annealing and extension , thereby increasing the overall stringency of the reaction and minimizing the extension of primers that ...
Page 8
... temperatures as quickly as possible . In the current instrument , this results in a heating rate of about 0.3 ° C per sec and a cooling rate of about 1 ° C per sec , for an overall single cycle time of approximately 3.75 min . ) These ...
... temperatures as quickly as possible . In the current instrument , this results in a heating rate of about 0.3 ° C per sec and a cooling rate of about 1 ° C per sec , for an overall single cycle time of approximately 3.75 min . ) These ...
Page 11
... temperatures corresponding to the three steps in a cycle of amplification - denaturation , annealing , and extension . This cycling can be accomplished either manually with pre - set water baths , or automatically with the DNA Thermal ...
... temperatures corresponding to the three steps in a cycle of amplification - denaturation , annealing , and extension . This cycling can be accomplished either manually with pre - set water baths , or automatically with the DNA Thermal ...
Page 13
... temperature to another , depends on the type of equipment used . With some notable exceptions , this rate of temperature change is not important and the fastest ramps attainable are used to shorten the cycle time . However , in order to ...
... temperature to another , depends on the type of equipment used . With some notable exceptions , this rate of temperature change is not important and the fastest ramps attainable are used to shorten the cycle time . However , in order to ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube