PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 2
... tube . The effect of varying the reaction parameters ( e.g. , enzyme , primer and Mg + concentration as well as the temperature cycling profile ) on the specificity and yield of the amplification is discussed in Chapter 1. Although ...
... tube . The effect of varying the reaction parameters ( e.g. , enzyme , primer and Mg + concentration as well as the temperature cycling profile ) on the specificity and yield of the amplification is discussed in Chapter 1. Although ...
Page 14
... tube to equilibrate with the block temperature . ) In some cases , primers of only 12 to 15 bases are available and an annealing temperature around 40-45 ° C is needed . However , primers of that length are unlikely to remain annealed ...
... tube to equilibrate with the block temperature . ) In some cases , primers of only 12 to 15 bases are available and an annealing temperature around 40-45 ° C is needed . However , primers of that length are unlikely to remain annealed ...
Page 18
... tube ) is 95 ° C for 20 sec in each cycle . Taq DNA polymerase activity is sensitive to the concentration of magnesium ion as well as to the nature and concentration of monovalent ions ( DHG , in preparation ) . Using minimally ...
... tube ) is 95 ° C for 20 sec in each cycle . Taq DNA polymerase activity is sensitive to the concentration of magnesium ion as well as to the nature and concentration of monovalent ions ( DHG , in preparation ) . Using minimally ...
Page 24
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube