PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Page 1
... cloning , pulsed field gel electrophoresis ) has often transformed the way we think about approaching both fundamental and applied biological problems . The capacity to amplify specific segments of DNA , made possible by the polymerase ...
... cloning , pulsed field gel electrophoresis ) has often transformed the way we think about approaching both fundamental and applied biological problems . The capacity to amplify specific segments of DNA , made possible by the polymerase ...
Page 2
... cloning a ẞ - globin amplification reaction and screening the individual clones with a ẞ - globin probe to detect the target sequence and with one of primers to detect any amplified sequence , the specificity of the PCR was estimated to ...
... cloning a ẞ - globin amplification reaction and screening the individual clones with a ẞ - globin probe to detect the target sequence and with one of primers to detect any amplified sequence , the specificity of the PCR was estimated to ...
Page 3
... clones generated by cloning the products of a particular amplification reaction . In this initial study , the frequency of errors was found to be ~ 1 / 400 and the error rate * After the first few cycles , virtually all of the templates ...
... clones generated by cloning the products of a particular amplification reaction . In this initial study , the frequency of errors was found to be ~ 1 / 400 and the error rate * After the first few cycles , virtually all of the templates ...
Page 4
... clones derived from a PCR , sequences must be determined from multiple clones to distinguish misincorporated nucleotides from the faithful copes of the template sequence . Other DNA polymerases with an active " proof - reading ...
... clones derived from a PCR , sequences must be determined from multiple clones to distinguish misincorporated nucleotides from the faithful copes of the template sequence . Other DNA polymerases with an active " proof - reading ...
Page 5
... clones by conferring on a specific sequence the ability to replicate by inserting it into a vector and introducing the vector into a host cell . PCR represents a form of " in vitro cloning " that can generate , as well as modify , DNA ...
... clones by conferring on a specific sequence the ability to replicate by inserting it into a vector and introducing the vector into a host cell . PCR represents a form of " in vitro cloning " that can generate , as well as modify , DNA ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube