PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Page ii
... molecular biology ) 1. DNA polymerases . 2. DNA - Synthesis . 3. DNA polymerases - Biotechnology . I. Erlich , Henry A. , 1943- . II . Series . [ DNLM : 1. DNA Polymerases . 2. Gene Amplification . 3. Genetic Engineering . 4. Molecular ...
... molecular biology ) 1. DNA polymerases . 2. DNA - Synthesis . 3. DNA polymerases - Biotechnology . I. Erlich , Henry A. , 1943- . II . Series . [ DNLM : 1. DNA Polymerases . 2. Gene Amplification . 3. Genetic Engineering . 4. Molecular ...
Page v
... Molecular Biology Department of Biological Sciences University of Southern California Edward T. Blake , D. Crim ... Molecular Genetics Investigator , Howard Hughes Institute Baylor College of Medicine J.S. Chamberlain , Ph.D. Post ...
... Molecular Biology Department of Biological Sciences University of Southern California Edward T. Blake , D. Crim ... Molecular Genetics Investigator , Howard Hughes Institute Baylor College of Medicine J.S. Chamberlain , Ph.D. Post ...
Page vi
... Molecular Genetics Baylor College of Medicine Ulf Gyllensten , Ph.D. Department of Medical Genetics Biomedical Center , Uppsala , Sweden J. Han , Ph.D. Research Fellow Department of Cellular and Molecular Biology Merck , Sharp and Dohme ...
... Molecular Genetics Baylor College of Medicine Ulf Gyllensten , Ph.D. Department of Medical Genetics Biomedical Center , Uppsala , Sweden J. Han , Ph.D. Research Fellow Department of Cellular and Molecular Biology Merck , Sharp and Dohme ...
Page vii
... Molecular Biology Merck , Sharp and Dohme Research Laboratories G.E. Mark , Ph.D. Associate Director Department of Cellular and Molecular Biology Merck , Sharp and Dohme Research Laboratories Richard M. Myers , Ph.D. Assistant Professor ...
... Molecular Biology Merck , Sharp and Dohme Research Laboratories G.E. Mark , Ph.D. Associate Director Department of Cellular and Molecular Biology Merck , Sharp and Dohme Research Laboratories Richard M. Myers , Ph.D. Assistant Professor ...
Page viii
... Molecular Biology Merck , Sharp and Dohme Research Laboratories Cecilia H. von Beroldingen , Ph.D. Post - doctoral Fellow Department of Public Health University of California , Berkeley Alice Wang Associate Scientist Department of Molecular ...
... Molecular Biology Merck , Sharp and Dohme Research Laboratories Cecilia H. von Beroldingen , Ph.D. Post - doctoral Fellow Department of Public Health University of California , Berkeley Alice Wang Associate Scientist Department of Molecular ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube