PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Page ix
... Mutation Detection by PCR , GC - Clamps , and Denaturing Gradient Gel Electrophoresis 71 Richard M. Myers , Val C. Sheffield , and David R. Cox 8 . Detection of Gene Expression 89 Ernest S. Kawasaki and Alice M. Wang 9 . PCR ...
... Mutation Detection by PCR , GC - Clamps , and Denaturing Gradient Gel Electrophoresis 71 Richard M. Myers , Val C. Sheffield , and David R. Cox 8 . Detection of Gene Expression 89 Ernest S. Kawasaki and Alice M. Wang 9 . PCR ...
Page x
... Mutation Diseases Using the Polymerase Chain Reaction 171 R.A. Gibbs , J.S. Chamberlain , and C. Thomas Caskey 16 . HLA Class II Gene Polymorphism : DNA Typing , Evolution , and Relationship to Disease Susceptibility 193 Henry A. Erlich ...
... Mutation Diseases Using the Polymerase Chain Reaction 171 R.A. Gibbs , J.S. Chamberlain , and C. Thomas Caskey 16 . HLA Class II Gene Polymorphism : DNA Typing , Evolution , and Relationship to Disease Susceptibility 193 Henry A. Erlich ...
Page 3
... mutations , changes introduced into the sequence of the PCR products due to nucleotide misincorporation can create potential problems . An estimate of the fidelity of Taq polymerase during the PCR was made by the sequence analysis of ...
... mutations , changes introduced into the sequence of the PCR products due to nucleotide misincorporation can create potential problems . An estimate of the fidelity of Taq polymerase during the PCR was made by the sequence analysis of ...
Page 39
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Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube