PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
From inside the book
Page 1
... strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in the exponential ...
... strands and flank the region of interest in the target DNA . A repetitive series of cycles involving template denaturation , primer annealing , and the extension of the annealed primers by DNA polymerase results in the exponential ...
Page 2
... strands at the outset of each PCR cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the thermostable DNA polymerase ( Taq polymerase ) isolated from Thermus aquaticus ( see Chapter 2 ) ...
... strands at the outset of each PCR cycle . Consequently , fresh enzyme had to be added during every cycle . The introduction of the thermostable DNA polymerase ( Taq polymerase ) isolated from Thermus aquaticus ( see Chapter 2 ) ...
Page 3
... strands at high product concentration may also contribute to the plateau effect and are discussed in Chapter 1. In addition to the increase in the specificity and yield of PCR made possible by Taq polymerase , the use of this enzyme ...
... strands at high product concentration may also contribute to the plateau effect and are discussed in Chapter 1. In addition to the increase in the specificity and yield of PCR made possible by Taq polymerase , the use of this enzyme ...
Page 14
... strand separation occurs . A temperature of about 94 ° C should be adequate in most cases . As soon as the sample reaches 94 ° C , it can be cooled to the annealing temperature . Extensive denaturation is probably unnecessary and ...
... strand separation occurs . A temperature of about 94 ° C should be adequate in most cases . As soon as the sample reaches 94 ° C , it can be cooled to the annealing temperature . Extensive denaturation is probably unnecessary and ...
Page 17
... strand separation , the tedium and frequent rebellion resulting from having to add Poll - Kf after each cycle is minimized . ENZYMATIC CHARACTERISTICS AND PROPERTIES T. aquaticus strain YT1 , a thermophilic , eubacterial microorganism ...
... strand separation , the tedium and frequent rebellion resulting from having to add Poll - Kf after each cycle is minimized . ENZYMATIC CHARACTERISTICS AND PROPERTIES T. aquaticus strain YT1 , a thermophilic , eubacterial microorganism ...
Contents
1 | |
9 | |
17 | |
PCR Automation | 23 |
Simple and Rapid Preparation | 31 |
PART TWO RESEARCH APPLICATIONS | 39 |
Using PCR to Engineer DNA | 61 |
Mutation Detection by PCR GCClamps | 71 |
The Use of Repeat Sequence | 113 |
A New Approach to Constructing Genetic | 119 |
Evolutionary Analysis via PCR | 137 |
PART THREE MEDICAL APPLICATIONS | 149 |
Diagnosis of New Mutation Diseases Using | 171 |
Applications of PCR to the Analysis | 209 |
Detection of ras Oncogenes Using PCR | 225 |
Application of PCR to the Detection | 235 |
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp gel electrophoresis Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube