Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 416
... Cotton effects ( see Chapter 23 , this volume , Fig . 22 ) . Poly - L - histidine at low pH , where it shows properties of the random coil , displays a positive Cotton effect at about 220 nm . However , in the helical form at pH 5.78 ...
... Cotton effects ( see Chapter 23 , this volume , Fig . 22 ) . Poly - L - histidine at low pH , where it shows properties of the random coil , displays a positive Cotton effect at about 220 nm . However , in the helical form at pH 5.78 ...
Page 417
... Cotton effects centered at 274 and 228 nm , and these disappear when the protein is dissociated into subunits in 6 M guanidine hydrochloride . Streptavidin shows similar Cotton effects , both proteins exhibiting a peak instead of a ...
... Cotton effects centered at 274 and 228 nm , and these disappear when the protein is dissociated into subunits in 6 M guanidine hydrochloride . Streptavidin shows similar Cotton effects , both proteins exhibiting a peak instead of a ...
Page 420
... Cotton effects to date and reveals the special powers of the technique in re- vealing subtle conformational changes which involve interactions with chromophoric residues . The sign and position of Cotton effects of di- sulfides are also ...
... Cotton effects to date and reveals the special powers of the technique in re- vealing subtle conformational changes which involve interactions with chromophoric residues . The sign and position of Cotton effects of di- sulfides are also ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure band Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient complex conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein solution pulse radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero