Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 19
... derived density p'D for the solvent components in the protein solution after c2 is measured . The derived density is obtained by subtracting the number of grams per milliliter of protein from the observed density ( giving c1 + c1 ) and ...
... derived density p'D for the solvent components in the protein solution after c2 is measured . The derived density is obtained by subtracting the number of grams per milliliter of protein from the observed density ( giving c1 + c1 ) and ...
Page 22
... derived from density measurements is dis- cussed in Section IV , B . The volume change can be obtained indirectly from the effect of pressure on the equilibrium constant of a reaction AV = -RT ( din K ) ( dmK ) dP ( 30 ) where K is the ...
... derived from density measurements is dis- cussed in Section IV , B . The volume change can be obtained indirectly from the effect of pressure on the equilibrium constant of a reaction AV = -RT ( din K ) ( dmK ) dP ( 30 ) where K is the ...
Page 570
... derived from these . The prominent aspect is a negative band at 217 nm , observed in all immunoglobulins ( see also , for example , Cathou et al . , 1968 ; Ikeda et al . , 1968 ; Ross and Jirgensons , 1968 ; Ghose and Jirgensons , 1971 ...
... derived from these . The prominent aspect is a negative band at 217 nm , observed in all immunoglobulins ( see also , for example , Cathou et al . , 1968 ; Ikeda et al . , 1968 ; Ross and Jirgensons , 1968 ; Ghose and Jirgensons , 1971 ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure band Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient complex conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein solution pulse radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero