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a-helix absorption acid angle axial ratio Biol calculated CD spectra Chem chromophore circular dichroism coefficient Cohn complex conformation constant contribution corresponding Cotton effects creatine kinase denaturation determined dialyzate diffusible components dilute electron density enzyme equation equilibrated equilibrium exciton experimental frequency function gm/ml grams helical helix hemocyanin hemoglobin intensity interaction isomolal kinase Kratky macromolecule magnetic mass measurements membrane method milliliter mixture molal molecular weight molecule myoglobin negative nondiffusible number of moles observed obtained optical activity osmometers osmotic pressure paramagnetic parameter partial specific volume peptide Phys Pilz plot polymer polypeptides positive primary beam procedure protein component protein concentration protein solution pulse radius of gyration random coil residues rotational strength sample Scatchard Schellman Section shape small-angle scattering solvent solvent medium spectrum sphere structure studies subunits techniques temperature theoretical scattering curves tion transition volume change wavelength zero