Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 56
... tion into a weighed amount of dialyzate . Since the densities in each set of components are known from the density measurements after equilibration , the volume dilution is known almost exactly by Volume dilution = ( E ) / + ( 2 ) / gi ...
... tion into a weighed amount of dialyzate . Since the densities in each set of components are known from the density measurements after equilibration , the volume dilution is known almost exactly by Volume dilution = ( E ) / + ( 2 ) / gi ...
Page 316
... tion VI . IV . Statistical Aspects A. CHOICE OF WEIGHTING FUNCTION i i Factors affecting the choice of an appropriate weight W , for the de- viation 8 have been discussed by Hamilton ( 1964 ) and Blackburn ( 1970 ) . A commonly used ...
... tion VI . IV . Statistical Aspects A. CHOICE OF WEIGHTING FUNCTION i i Factors affecting the choice of an appropriate weight W , for the de- viation 8 have been discussed by Hamilton ( 1964 ) and Blackburn ( 1970 ) . A commonly used ...
Page 437
... tion bands which give rise to only weak optical activity . In these cases , since CD can only be measured at the same wavelength as the absorp- tion bands , the concentration of the molecule cannot be increased to im- prove the signal ...
... tion bands which give rise to only weak optical activity . In these cases , since CD can only be measured at the same wavelength as the absorp- tion bands , the concentration of the molecule cannot be increased to im- prove the signal ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure band Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient complex conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein solution pulse radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero