Systematic Approach to Evaluation of Mouse MutationsJohn P. Sundberg Experts from The Jackson Laboratory and around the world provide practical advice on everything from how to establish a colony to where to go for specific mutations. The book includes information on medical photography, grafting procedures, and how to map the genes and evaluate the special biological characteristics of mice. It also discusses how to maintain a colony of mice that breed with difficulty, how to approach mapping spontaneous mutations, how to set up systems to evaluate a specific antibody, how to perform simple measurements that yield a large amount of information, and how to access mouse informatics on the Web. |
Contents
Chapter 3 Computerized Colony Management | |
Chapter 4 Medical Record Keeping for Project Analysis | viii |
Biological Characterization of a New Mutation | xvii |
Chapter 6 Photography of Laboratory Mice | i |
Chapter 7 Comparative Pathology and Animal Model Development | xi |
Chapter 9 Ultrastructural Evaluation of Mouse Mutations | xxix |
Chapter 10 Immunohistochemical and Immunofluorescence Methods | xxxviii |
Chapter 11 Radiolabeled cRNA and In Situ Hybridization | 2 |
Chapter 12 Use of Nonradiolabeled Probes for In Situ Hybridization | 24 |
Chapter 13 Repositories of Mouse Mutations and Inbred Congenic and Recombinant Inbred Strains | 34 |
Index | 191 |
Back cover | 200 |
Chapter 8 Kinetics and Morphometrics | xx |
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Common terms and phrases
abnormal added additional affected allow analysis animal antibody application background become blocking blood breeding buffer cage camera cells changes chromosome collected colony color compared completely concentration containing cRNA cross database determine disease electron emulsion ethanol evaluation example experiment expression Figure final fixation fixative forceps gene genetic Genome genotyped glass hair histological human hybridization identify images important inbred strains individual known labeled Laboratory light maintained mapping markers matings method mice microscope mouse mouse mutation mutation necessary needed normal observed obtained organs ovary phenotype photographic placed position possible prepared present probes procedure processing produce progeny protocol record REFERENCES removed Resource Room temperature side skin slides solution specific specimen step sterile stored strains Sundberg Table techniques tissue transplanted trimmed types usually Wash
Popular passages
Page xxi - Threadgill, DW, Dlugosz, AA, Hansen, LA, Tennenbaum, T., Lichti, U., Yee, D., LaMantia, C., Mourton, T., Herrup, K., Harris, RC, Barnard, JA, Yuspa, S. H., Coffey, RJ, and Magnuson, T. (1995) Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype.
Page xxix - RM (1990) Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell...
Page 27 - Sambrook, J., Fritsch, EF, and Maniatis, T., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
Page 194 - Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice. Proc. Natl. Acad. Sci. USA 93, 10,933-10,938.