Physical Principles and Techniques of Protein Chemistry Part A, Part 1Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 74
Page 60
... first kind of order n and argument 2: I. Introduction X—Ray diffraction methods are assuming an increasingly important role in studies of protein structure and function. The aims of the present chapter are to provide an introduction to ...
... first kind of order n and argument 2: I. Introduction X—Ray diffraction methods are assuming an increasingly important role in studies of protein structure and function. The aims of the present chapter are to provide an introduction to ...
Page 79
... first synthesis bears no obvious relation to the structure While the second is a surprisingly good representation. Further examples of the overwhelming importance of phases may be found in an article by Ramachandran (1964). B. TRIAL-AND ...
... first synthesis bears no obvious relation to the structure While the second is a surprisingly good representation. Further examples of the overwhelming importance of phases may be found in an article by Ramachandran (1964). B. TRIAL-AND ...
Page 83
... first synthesis. The coordinates of these atoms are not quite correct but the improvement in the calculated phases ac(hkl) results in a more accurate map, as will be seen from Fig. 160. Further cycles of refinement may be carried out ...
... first synthesis. The coordinates of these atoms are not quite correct but the improvement in the calculated phases ac(hkl) results in a more accurate map, as will be seen from Fig. 160. Further cycles of refinement may be carried out ...
Page 85
... first stage is the elucidation of the position of the heavy atom. This would be very difficult to deduce from a Patterson map calculated according to Eq. (12) because the peaks due to heavy atom~heavy atom vectors would be hard to find ...
... first stage is the elucidation of the position of the heavy atom. This would be very difficult to deduce from a Patterson map calculated according to Eq. (12) because the peaks due to heavy atom~heavy atom vectors would be hard to find ...
Page 94
... first, by Cochran et al. (1952), an expression was obtained for calculating the Fourier transform of a helix and in the second, by Klug et al. (1958), various extensions of the theory are discussed. In the case of a paracrystalline ...
... first, by Cochran et al. (1952), an expression was obtained for calculating the Fourier transform of a helix and in the second, by Klug et al. (1958), various extensions of the theory are discussed. In the case of a paracrystalline ...
Contents
59 | |
Chapter 3 Ultraviolet Absorption | 101 |
Chapter 4 Fluorescence of Proteins | 171 |
Chapter 5 Perturbation and Flow Techniques | 245 |
Chapter 6 Dielectric Properties of Proteins I Dielectric Relaxation | 291 |
Chapter 7 Dielectric Properties of Proteins II Electric Birefringence and Dichroism | 335 |
Chapter 8 Electrophoresis | 369 |
Chapter 9 Analytical Gel Filtration | 451 |
Author Index | 497 |
Subject Index | 509 |
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient concentration curve defined denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction diffusion dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor field strength film filters first flow fluorescence fraction frequency gel filtration groups intensity interactions ionic strength ions light macromolecules magnification measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic phenylalanine photomultiplier Phys plot polarization polymer protein quantum yield ratio reaction reflections relaxation residues ribonuclease rotation shown in Fig significant solution solvent specific specimen spectra structure sufficiently technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone