Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 61
Page 27
Sydney Leach. FIG . 13. Absorption sedimentation velocity photograph and photodensitometer trace . 1 , Inner reference edge ; 2 , air - solution meniscus ; 3 , boundary ; 4 , outer reference edge . low concentrations and in the case of ...
Sydney Leach. FIG . 13. Absorption sedimentation velocity photograph and photodensitometer trace . 1 , Inner reference edge ; 2 , air - solution meniscus ; 3 , boundary ; 4 , outer reference edge . low concentrations and in the case of ...
Page 33
... Absorption Optical System Photographs . These photographs require measurement with a photodensitometer that is ca- pable of measuring the extent of blackening across the photographic image . It is important that the instrument be linear ...
... Absorption Optical System Photographs . These photographs require measurement with a photodensitometer that is ca- pable of measuring the extent of blackening across the photographic image . It is important that the instrument be linear ...
Page 68
... absorption by solutes in the ultracentrifuge cell raises the possibility of measuring directly and simultaneously the concentration distribution of two interacting solutes . Since thermodynamic equilibrium exists at all 68 J. H. COATES.
... absorption by solutes in the ultracentrifuge cell raises the possibility of measuring directly and simultaneously the concentration distribution of two interacting solutes . Since thermodynamic equilibrium exists at all 68 J. H. COATES.
Page 81
... concentration of protein needed for a successful experiment using schlieren optics is much greater than the concentration of nucleic acid required when absorption dn dx dn dx 82 J. H. COATES X- > 10. ULTRACENTRIFUGAL ANALYSIS 81.
... concentration of protein needed for a successful experiment using schlieren optics is much greater than the concentration of nucleic acid required when absorption dn dx dn dx 82 J. H. COATES X- > 10. ULTRACENTRIFUGAL ANALYSIS 81.
Page 83
... absorption optical system . There are practical limitations to the use of very high rotor speeds with high density solutions . For the most precise work using schlieren optics it is desirable to use double sector synthetic boundary ...
... absorption optical system . There are practical limitations to the use of very high rotor speeds with high density solutions . For the most precise work using schlieren optics it is desirable to use double sector synthetic boundary ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-ยน coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero