Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Results 6-10 of 77
Page 32
... calculated, suggesting that the structure of the activated, monomer—seed polymer complex was similar to that of the components before the activation. Above 28°C, the decreasing rate of polymerization and higher maximum AV were ascribed ...
... calculated, suggesting that the structure of the activated, monomer—seed polymer complex was similar to that of the components before the activation. Above 28°C, the decreasing rate of polymerization and higher maximum AV were ascribed ...
Page 41
... calculated from the square of the observed voltage and the slope and intercept values of the rectilinear calibration ... calculate v°' and w, respectively (i.e., confidences relative to the densities assigned to the reference 17. DENSITY ...
... calculated from the square of the observed voltage and the slope and intercept values of the rectilinear calibration ... calculate v°' and w, respectively (i.e., confidences relative to the densities assigned to the reference 17. DENSITY ...
Page 56
... calculated by p” = p'(1 — (#202) + 02 (47) since p' = c'1 + 0'3 and p” = c”1 + 02 +c"a = (c'1 + 0'3) (1 — ¢202) + 02 [cf. Eqs. (43)]. The values of 81 and 83 are then obtained with Eqs. (41) by substituting the values of {1'1 and v ...
... calculated by p” = p'(1 — (#202) + 02 (47) since p' = c'1 + 0'3 and p” = c”1 + 02 +c"a = (c'1 + 0'3) (1 — ¢202) + 02 [cf. Eqs. (43)]. The values of 81 and 83 are then obtained with Eqs. (41) by substituting the values of {1'1 and v ...
Page 57
... calculation of 81 and 8,, evaluation Of £1 and $3 is then carried out with the use Of Eq. (45). 5 may also be calculated from a determination Of the components by dry weight analysis on the protein solution and dialyzate. If component 3 ...
... calculation of 81 and 8,, evaluation Of £1 and $3 is then carried out with the use Of Eq. (45). 5 may also be calculated from a determination Of the components by dry weight analysis on the protein solution and dialyzate. If component 3 ...
Page 58
... calculated from a determination of v°', or vice verse, by (1 — 'P') — (1 — W'p') = 51°(1 - V'm') (j = 1, 3) (49) which follows by substituting from Eqs. (18) and (12) into Eq. (48). In terms of the corresponding apparent quantities ...
... calculated from a determination of v°', or vice verse, by (1 — 'P') — (1 — W'p') = 51°(1 - V'm') (j = 1, 3) (49) which follows by substituting from Eqs. (18) and (12) into Eq. (48). In terms of the corresponding apparent quantities ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero