Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 86
Page 15
... constant , yields an equa- tion for the weight - average molecular weight of the protein solutes re- ferring to the original solution M = w 2RT ( cb - Ca ) Co ( xb2x2 ) ( ap / ac ) μ ° ( 12 ) The term ( ap / dc ) μ ° is the density ...
... constant , yields an equa- tion for the weight - average molecular weight of the protein solutes re- ferring to the original solution M = w 2RT ( cb - Ca ) Co ( xb2x2 ) ( ap / ac ) μ ° ( 12 ) The term ( ap / dc ) μ ° is the density ...
Page 16
... constant concentration of added electrolyte will eliminate the effect , provided that there are no specific interactions be- tween the protein and any of the electrolyte ions . The secondary salt effect occurs if the sedimentation ...
... constant concentration of added electrolyte will eliminate the effect , provided that there are no specific interactions be- tween the protein and any of the electrolyte ions . The secondary salt effect occurs if the sedimentation ...
Page 39
... constant through the range of concentrations encountered . i c . Calculations and the Use of a Computer . The sedimentation coeffi- cient for a velocity run is calculated from the slope of a plot of log x or log against time . A ...
... constant through the range of concentrations encountered . i c . Calculations and the Use of a Computer . The sedimentation coeffi- cient for a velocity run is calculated from the slope of a plot of log x or log against time . A ...
Page 42
... constant . C. DETECTION OF HETEROGENEITY 1. Sedimentation Coefficient Distributions The appearance of two or more distinct peaks in a sedimentation velocity schlieren pattern indicates the presence of several solutes with distinctly ...
... constant . C. DETECTION OF HETEROGENEITY 1. Sedimentation Coefficient Distributions The appearance of two or more distinct peaks in a sedimentation velocity schlieren pattern indicates the presence of several solutes with distinctly ...
Page 44
... constant expressed in ergs mole - 1 degree - 1 . If the intrinsic viscosity is measured , the Scheraga and Mandelkern ( 1953 ) theory may be employed , but the Svedberg equation is in principle to be preferred in that its derivation by ...
... constant expressed in ergs mole - 1 degree - 1 . If the intrinsic viscosity is measured , the Scheraga and Mandelkern ( 1953 ) theory may be employed , but the Svedberg equation is in principle to be preferred in that its derivation by ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero