Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Results 6-10 of 90
Page 8
... definition for this volume quantity, Eq. (2), we wish to measure the change in volume of a solution upon addition of an infinitesimal amount of the component in question. This can be done most conveniently by measuring the densities of ...
... definition for this volume quantity, Eq. (2), we wish to measure the change in volume of a solution upon addition of an infinitesimal amount of the component in question. This can be done most conveniently by measuring the densities of ...
Page 9
... defined at a finite concentration of any component i by including a specified mass of that component in the initial solution; in effect, the solvent medium of density p' already contains the component for which a. is desired at ...
... defined at a finite concentration of any component i by including a specified mass of that component in the initial solution; in effect, the solvent medium of density p' already contains the component for which a. is desired at ...
Page 10
... defined to include a stoichiometric number of small ions of opposite charge in addition to the protein ion. When this component is added to an unbufi'ered solvent medium, the change in pH with concentration of component 2 may be ...
... defined to include a stoichiometric number of small ions of opposite charge in addition to the protein ion. When this component is added to an unbufi'ered solvent medium, the change in pH with concentration of component 2 may be ...
Page 11
... definition, is the difference in volume between that Of the solution and the solvent medium containing none of the ... defined in these terms by rearranging and by multiplying through by g, so that N V = (Pig; + 2 77'1"ng (16) .7741 ...
... definition, is the difference in volume between that Of the solution and the solvent medium containing none of the ... defined in these terms by rearranging and by multiplying through by g, so that N V = (Pig; + 2 77'1"ng (16) .7741 ...
Page 13
... defined on the basis of constant composition of the nonprotein components. In order to determine 952 at each 02, information is required on the changing mass ratios of the difiusible components in each equilibrated protein solution of ...
... defined on the basis of constant composition of the nonprotein components. In order to determine 952 at each 02, information is required on the changing mass ratios of the difiusible components in each equilibrated protein solution of ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero