Culturing Nerve CellsGary Banker, Kimberly Goslin A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. Because neurons and glia in culture are remarkably similar to those in situ, culture systems make it possible to identify significant cell interactions and to elucidate their mechanisms. This book is in many ways a do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. But it also provides an understanding of the principles behind the protocols. In effect the contributors invite you into their labs and provide much of the information you would obtain from such a visit.The authors of the introductory chapters present the nuts-and-bolts principles of growing nerve cells. The authors of the following chapters discuss the culturing of specific cell types. They explain how their experimental goals have shaped their particular cell culture approach and the advantages and disadvantages of the cell culture systems they have developed. They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development. Contributors |
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Contents
Getting Started | 3 |
Types of Nerve Cell Cultures Their Advantages and Limitations | 11 |
Primary Dissociated Cell Cultures | 37 |
Transfecting Cultured Neurons | 79 |
Characterizing and Studying Neuronal Cultures | 113 |
Choosing the Right System | 155 |
Neuronlike Cells Derived in Culture from P19 Embryonal Carcinoma | 189 |
Culturing the Large Neurons of Aplysia | 213 |
Mass Cultures and Microislands of Neurons from Postnatal Rat Brain | 309 |
Rat Hippocampal Neurons in LowDensity Culture | 339 |
Rat Striatal Neurons in LowDensity SerumFree Culture | 371 |
Cell Culture of Cholinergic and Cholinoceptive Neurons from the Medial | 395 |
Purification and Coculture of Identified Cell Populations | 419 |
Organotypic Slice Cultures of Neural Tissue | 461 |
Culture of Astrocytes Oligodendrocytes and 02A Progenitor Cells | 499 |
Tissue Culture Methods for the Study of Myelination | 545 |