In Vitro Biological Systems: Methods in Toxicology, Vol. 1, Volume 1Charles A. Tyson, John M. Frazier Methods in Toxicology, Volume 1: In Vitro Biological Systems, Part A provides basic techniques employed by widely recognized scientists to prepare and maintain the biological components of in vitro model systems. The book discusses the in vitro models of neural and neuromuscular systems; ocular system; respiratory system; cardiovascular system; and gastrointestinal system. The text also describes liver slices; liver hepatocytes; other liver cell systems; proximal tubule fragments; kidney cell culture; reproductive and developmental systems; immune system; and skin. Pharmacologists, toxicologists, cell biologists, physiologists, immunotoxicologists, and molecular toxicologists will find the book invaluable. |
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Page 59
... glucose and both horse and fetal calf serum. Media glucose utilization can be substantial in dense older cultures, so one advantage of a high glucose concentration is better maintenance of glucose availability between feedings. Our ...
... glucose and both horse and fetal calf serum. Media glucose utilization can be substantial in dense older cultures, so one advantage of a high glucose concentration is better maintenance of glucose availability between feedings. Our ...
Page 61
... glucose, 17.82 g D.L-alanine, 23.38 g NaCl, and 10.48 ml of 86% (w/w) phosphoric acid in demineralized, distilled water. The solution is sterilized by filtration through a 0.22-um filter (Millipore, Bedford, MA) and stored as 25-ml ...
... glucose, 17.82 g D.L-alanine, 23.38 g NaCl, and 10.48 ml of 86% (w/w) phosphoric acid in demineralized, distilled water. The solution is sterilized by filtration through a 0.22-um filter (Millipore, Bedford, MA) and stored as 25-ml ...
Page 62
... Glucose: A total of 100 ml of 15% glucose is made up by dissolving 15.0 g glucose in 100 ml of demineralized, distilled water, and 1.5-ml aliquots are stored at -20°C. Glutamine: Glutamine (GIBCO, Santa Clara, CA, 320-5030PE) comes as ...
... Glucose: A total of 100 ml of 15% glucose is made up by dissolving 15.0 g glucose in 100 ml of demineralized, distilled water, and 1.5-ml aliquots are stored at -20°C. Glutamine: Glutamine (GIBCO, Santa Clara, CA, 320-5030PE) comes as ...
Page 63
... glucose, and ascorbic acid with glutathione are added. The volume is then brought up to 50 ml with basal L-15 medium. The solution is mixed and warmed to 37°C prior to feeding the cultures. This medium is used when neurons are grown in ...
... glucose, and ascorbic acid with glutathione are added. The volume is then brought up to 50 ml with basal L-15 medium. The solution is mixed and warmed to 37°C prior to feeding the cultures. This medium is used when neurons are grown in ...
Page 72
... glucose, 5 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.4] Collagenase type I (Boehringer-Mannheim, Indianapolis, IN) Deoxyribonuclease (DNase) (Sigma, St. Louis, MO) Renografin 60 (Squibb, New Brunswick, NJ) ...
... glucose, 5 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.4] Collagenase type I (Boehringer-Mannheim, Indianapolis, IN) Deoxyribonuclease (DNase) (Sigma, St. Louis, MO) Renografin 60 (Squibb, New Brunswick, NJ) ...
Contents
1 | |
94 | |
RESPIRATORY SYSTEM | 110 |
CARDIOVASCULAR SYSTEM | 147 |
GASTROINTESTINAL SYSTEM | 182 |
LIVER SLICES | 222 |
LIVER HEPATOCYTES | 231 |
LIVER OTHER CELL SYSTEMS | 279 |
KIDNEY PROXIMAL TUBULE FRAGMENTS | 330 |
KIDNEY CELL CULTURE | 366 |
REPRODUCTIVE AND DEVELOPMENTAL SYSTEMS | 420 |
IMMUNE SYSTEM | 455 |
SKIN | 504 |
Index | 531 |
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Common terms and phrases
1993 by Academic Academic Press acid activity aliquots animals assay basal beaker Biochem Biol bovine serum buffer calcium cannula cell cultures cell suspension cell types cellular centrifuge tube chemical Clara cells coculture collagenase concentration containing cortical coverslips culture medium density digestion dissecting dissociation enzyme epithelial cells ethanol explants fetal filter flask forceps gentamicin GIBCO glucose gradient growth HBSS hepatocytes HEPES human incubated isolated kidney Kupffer cells laboratory layer lipocytes liver lymphocytes macrophages membrane metabolism METHODS IN TOXICOLOGY mg/ml microscope monolayer mouse neurons Pasteur pipette pellet Percoll perfusion petri dish Pharmacol Physiol pipette plastic plates preparation procedure protein proximal tubule rabbit reaggregates receptor removed renal resuspended RPMI scissors Sertoli cells Sigma skin slices sodium specific sterile stock solution studies supernatant tion tissue culture toxicity Toxicol trypan blue trypsin type II cells ug/ml vessel viability vitro vivo washed xenobiotics