Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 41
... magnetic force emanating below the cell, as shown in the figure. The solenoid current I is proportional to the moment within very narrow limits for high permeability materials, and it is strictly proportional to the field gradient ...
... magnetic force emanating below the cell, as shown in the figure. The solenoid current I is proportional to the moment within very narrow limits for high permeability materials, and it is strictly proportional to the field gradient ...
Page 42
... magnetic densimetry is under continuing development. Efforts are being made to eliminate the need for multiple buoys and to cancel the magnetic moment so that the current is strictly proportional to the field gradient only (Beams, 1969) ...
... magnetic densimetry is under continuing development. Efforts are being made to eliminate the need for multiple buoys and to cancel the magnetic moment so that the current is strictly proportional to the field gradient only (Beams, 1969) ...
Page 50
... magnetic densimetry).4 In passing, we take note of the fact that the partial specific volume of a mixture of substances can be determined as well as that of a pure substance. The partial specific volume, or equivalently (Zip/8c.)m, of a ...
... magnetic densimetry).4 In passing, we take note of the fact that the partial specific volume of a mixture of substances can be determined as well as that of a pure substance. The partial specific volume, or equivalently (Zip/8c.)m, of a ...
Page 58
... magnetic densimeter on the dialyzate and on all of the diluted nucleoprotein solutions, yielding the data shown in the lower curve of density versus 02 in Fig. 4. The other half of the stock TYMV—water solution was made isomolal with ...
... magnetic densimeter on the dialyzate and on all of the diluted nucleoprotein solutions, yielding the data shown in the lower curve of density versus 02 in Fig. 4. The other half of the stock TYMV—water solution was made isomolal with ...
Page 60
... magnetic densimeter method (which is the technique most familiar to the writer), it is clear that the change in density with temperature may be followed easily and precisely through the use of a variable temperature controller.7 The ...
... magnetic densimeter method (which is the technique most familiar to the writer), it is clear that the change in density with temperature may be followed easily and precisely through the use of a variable temperature controller.7 The ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero