Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 93
Page 29
... Measuring Plates For precise work , both schlieren and Rayleigh interference plates should be measured on a measuring microscope or microcomparator with a stage allowing measurements in two dimensions and capable of being rotated ...
... Measuring Plates For precise work , both schlieren and Rayleigh interference plates should be measured on a measuring microscope or microcomparator with a stage allowing measurements in two dimensions and capable of being rotated ...
Page 30
... measured in the y - direction . For the measurement of unsymmetrical peaks , multiple peaks , or sedi- mentation equilibrium patterns , it is necessary to determine the height of the trace across the plate . For all precise work it is ...
... measured in the y - direction . For the measurement of unsymmetrical peaks , multiple peaks , or sedi- mentation equilibrium patterns , it is necessary to determine the height of the trace across the plate . For all precise work it is ...
Page 31
... measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are generated by ... measured along the y - coordinate to give the " fringe fraction . " The concentration difference between the ...
... measuring microscope by the use of " reference " fringes that appear at each end of the pattern , and which are generated by ... measured along the y - coordinate to give the " fringe fraction . " The concentration difference between the ...
Page 32
... measurement proce- dure . ( a ) Synthetic boundary pattern : 1 , inner reference fringes ; 2 , solution - air meniscus ; 3 ... measured from the plateau region toward the air - solution meniscus . In each case , the fringe fraction is ...
... measurement proce- dure . ( a ) Synthetic boundary pattern : 1 , inner reference fringes ; 2 , solution - air meniscus ; 3 ... measured from the plateau region toward the air - solution meniscus . In each case , the fringe fraction is ...
Page 33
... measurement of fringe positions even with a projection microscope can be tiring and tedious . A projection ... measuring the re- sistance of the photoresistor . This arrangement makes possible a less sub- jective assessment of the ...
... measurement of fringe positions even with a projection microscope can be tiring and tedious . A projection ... measuring the re- sistance of the photoresistor . This arrangement makes possible a less sub- jective assessment of the ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-ยน coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero