Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 78
... molal volume of component i volume of solution containing 1 kg of water at vanishing concentration of nondiifusible components weight fraction of component i relative to all components of a solution mole fraction of component i relative ...
... molal volume of component i volume of solution containing 1 kg of water at vanishing concentration of nondiifusible components weight fraction of component i relative to all components of a solution mole fraction of component i relative ...
Page 85
... molal free energy of the component, usually called the chemical potential and designated as m or (L. (The chemical potential may be defined by varying other thermodynamic energy quantities with the number of moles of matter. These ...
... molal free energy of the component, usually called the chemical potential and designated as m or (L. (The chemical potential may be defined by varying other thermodynamic energy quantities with the number of moles of matter. These ...
Page 88
... molal volume Vi, which is an intensive property of the phase and is independent of the size. Hence, the extra potential on the protein side required to maintain equilibrium is (1.4.1)pn plus the sum of each VldP in proceeding from P0 to ...
... molal volume Vi, which is an intensive property of the phase and is independent of the size. Hence, the extra potential on the protein side required to maintain equilibrium is (1.4.1)pn plus the sum of each VldP in proceeding from P0 to ...
Page 89
... molal concentrations or in weights of the components relative to a weight of water. At vanishing concentrations of protein, the mole fraction X2 becomes equal to nz/nl. The molality m2 of component 2, however, is 1000n2/M1n1), since ...
... molal concentrations or in weights of the components relative to a weight of water. At vanishing concentrations of protein, the mole fraction X2 becomes equal to nz/nl. The molality m2 of component 2, however, is 1000n2/M1n1), since ...
Page 93
... molal concentrations. The condition at osmotic equilibrium is then m'+m'_ = (m'i)2 = m+m_ (21) since ordinary pressure differences have no significant effect on the activities. But if Z2 is positive, the neutrality condition specifies ...
... molal concentrations. The condition at osmotic equilibrium is then m'+m'_ = (m'i)2 = m+m_ (21) since ordinary pressure differences have no significant effect on the activities. But if Z2 is positive, the neutrality condition specifies ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero