Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 98
... molecules are added to the system; hence, real nondiffusible molecules (having finite volumes) will show an increase in the amount of net repulsion as their concentration increases, even if they are chemically inert, because one molecule ...
... molecules are added to the system; hence, real nondiffusible molecules (having finite volumes) will show an increase in the amount of net repulsion as their concentration increases, even if they are chemically inert, because one molecule ...
Page 107
... molecule, or whether there is a separation of fragments at all after scission of peptide bonds. In other cases, diffusible fragments are produced so that the molecular weight of the nondiffusible material is lower than that of the ...
... molecule, or whether there is a separation of fragments at all after scission of peptide bonds. In other cases, diffusible fragments are produced so that the molecular weight of the nondiffusible material is lower than that of the ...
Page 108
... molecule was lower at all concentrations employed. While the difference in molecular weights was Within the ... molecules may be formed. The dissociation products or subunits, just discussed in Section III,B, were considered as though ...
... molecule was lower at all concentrations employed. While the difference in molecular weights was Within the ... molecules may be formed. The dissociation products or subunits, just discussed in Section III,B, were considered as though ...
Page 113
... molecules or to unlike in the sense of differences in the polypeptide chains, e.g., normal versus variant hemoglobins or to hemoglobins from different animal species.9 The existence of hybrid molecules of tetrameric hemoglobin in ...
... molecules or to unlike in the sense of differences in the polypeptide chains, e.g., normal versus variant hemoglobins or to hemoglobins from different animal species.9 The existence of hybrid molecules of tetrameric hemoglobin in ...
Page 117
... molecule of the protein plus five molecules of NaCl. By this means, the third term of Eq. (27 ) drops out if it is assumed also that the activity coefficient of the salt is otherwise independent of protein concentration. If the protein ...
... molecule of the protein plus five molecules of NaCl. By this means, the third term of Eq. (27 ) drops out if it is assumed also that the activity coefficient of the salt is otherwise independent of protein concentration. If the protein ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero