Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 41
Page 33
... density of photographic blackening over a small area of the plate may be ... Optical System Photographs . These photographs require measurement with a ... optical den- sity of the film ; this should be checked with calibrated neutral ...
... density of photographic blackening over a small area of the plate may be ... Optical System Photographs . These photographs require measurement with a ... optical den- sity of the film ; this should be checked with calibrated neutral ...
Page 34
... optical density of the solution is measured , since the nondialyzable components will determine the difference in prop- erties between the protein solution and the solvent . For many of the techniques in which the conservation of mass ...
... optical density of the solution is measured , since the nondialyzable components will determine the difference in prop- erties between the protein solution and the solvent . For many of the techniques in which the conservation of mass ...
Page 72
... optical density for the spectrophotometric deter- mination of concentration is 0.4 . If the initial protein solution has this optical density at the appropriate wavelength , the optical density at the zone maximum will decrease from ...
... optical density for the spectrophotometric deter- mination of concentration is 0.4 . If the initial protein solution has this optical density at the appropriate wavelength , the optical density at the zone maximum will decrease from ...
Page 75
... optical path length of 12 or 30 mm . Detailed instructions on how to handle ... density gradient , a con- ventional valve - type synthetic boundary cell ... density gradients the speed may be chosen to give the shortest convenient ...
... optical path length of 12 or 30 mm . Detailed instructions on how to handle ... density gradient , a con- ventional valve - type synthetic boundary cell ... density gradients the speed may be chosen to give the shortest convenient ...
Page 77
... density gradients should disappear if the run is carried out at lower speed ... density gradient , the density and the viscosity of the solvent must increase ... optical detection method is sufficiently sensitive , it may be possible to ...
... density gradients should disappear if the run is carried out at lower speed ... density gradient , the density and the viscosity of the solvent must increase ... optical detection method is sufficiently sensitive , it may be possible to ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-ยน coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero