Physical Principles and Techniques of Protein Chemistry Part A, Part 1Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Results 6-10 of 48
Page 84
... peaks, marked +, correspond to heavy atom—heavy atom vectors and from their positions the coordinates of the heavy atom may be deduced to be those shown in Fig. 15b. (b) A two-dimensional 1 A Fourier synthesis of the pleated-sheet ...
... peaks, marked +, correspond to heavy atom—heavy atom vectors and from their positions the coordinates of the heavy atom may be deduced to be those shown in Fig. 15b. (b) A two-dimensional 1 A Fourier synthesis of the pleated-sheet ...
Page 85
... peaks due to heavy atom~heavy atom vectors would be hard to find among such a dense population of interatomic vectors. Instead, various Patterson syntheses which depend on the differences between the parent protein and its derivatives ...
... peaks due to heavy atom~heavy atom vectors would be hard to find among such a dense population of interatomic vectors. Instead, various Patterson syntheses which depend on the differences between the parent protein and its derivatives ...
Page 86
... peaks show up much more clearly than in the ordinary synthesis in Fig. 16a. If sufficient centrosymmetric projections are present, all three heavy atom coordinates may be deduced from syntheses of the type shown in Fig. 17. If not, the ...
... peaks show up much more clearly than in the ordinary synthesis in Fig. 16a. If sufficient centrosymmetric projections are present, all three heavy atom coordinates may be deduced from syntheses of the type shown in Fig. 17. If not, the ...
Page 89
... accurate, the resolution is high and the experimental errors are small, weak positive peaks will appear in the positions occupied by the hydrogen atoms. The hydrogen atoms FIGURE 20a b 0 FIGURE 20b /=\ 07'?“ 0 its". 2. X-RAY METHODS 89.
... accurate, the resolution is high and the experimental errors are small, weak positive peaks will appear in the positions occupied by the hydrogen atoms. The hydrogen atoms FIGURE 20a b 0 FIGURE 20b /=\ 07'?“ 0 its". 2. X-RAY METHODS 89.
Page 90
... peaks correspond to the hydrogen atoms shown in Fig. 5. In (b) the contour interval is le/A2 and negative contours are broken. appear as positive peaks because they are not normally included. 90 R. D. B. FRASER AND T. P. MACRAE.
... peaks correspond to the hydrogen atoms shown in Fig. 5. In (b) the contour interval is le/A2 and negative contours are broken. appear as positive peaks because they are not normally included. 90 R. D. B. FRASER AND T. P. MACRAE.
Contents
59 | |
Chapter 3 Ultraviolet Absorption | 101 |
Chapter 4 Fluorescence of Proteins | 171 |
Chapter 5 Perturbation and Flow Techniques | 245 |
Chapter 6 Dielectric Properties of Proteins I Dielectric Relaxation | 291 |
Chapter 7 Dielectric Properties of Proteins II Electric Birefringence and Dichroism | 335 |
Chapter 8 Electrophoresis | 369 |
Chapter 9 Analytical Gel Filtration | 451 |
Author Index | 497 |
Subject Index | 509 |
Common terms and phrases
absorption absorption spectrum amino acids applied axis Biochem Biol Biophys birefringence boundary bovine serum albumin buffer calculated Cann Chem chromophores coefficient concentration curve defined denaturation density determined dielectric constant dielectric increment dielectric relaxation difference spectrum diffraction diffusion dipole moment Edelhoch effects electric birefringence electric field electron microscope electrophoresis elution volume emission energy enzyme equation equilibrium excitation experimental factor field strength film filters first flow fluorescence fraction frequency gel filtration groups intensity interactions ionic strength ions light macromolecules magnification measured method migration mobility molar molecular weight molecules moving-boundary observed obtained optical ovalbumin parameter particles peaks permanent dipole perturbation phase phenolic phenylalanine photomultiplier Phys plot polarization polymer protein quantum yield ratio reaction reflections relaxation residues ribonuclease rotation shown in Fig significant solution solvent specific specimen spectra structure sufficiently technique temperature theoretical theory tion tryptophan tyrosine unit cell values wavelength Weber Winzor zone