Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Results 6-10 of 49
Page 66
... reaction of the form A + B⇒C . ( a ) vc > và > VB , component A dominating . ( b ) vc > VA > VB , component B dominating . ( c ) vc = VA > VB . The positions marked va , vâ , vo show the distances that the components would have moved ...
... reaction of the form A + B⇒C . ( a ) vc > và > VB , component A dominating . ( b ) vc > VA > VB , component B dominating . ( c ) vc = VA > VB . The positions marked va , vâ , vo show the distances that the components would have moved ...
Page 67
Sydney Leach. and Ogston ( 1967 ) have also applied constituent concepts to reactions of the type A + B C + D. 2. Sedimentation Equilibrium a . The Application of Differential Equations When Interactions Exist . The equilibrium method ...
Sydney Leach. and Ogston ( 1967 ) have also applied constituent concepts to reactions of the type A + B C + D. 2. Sedimentation Equilibrium a . The Application of Differential Equations When Interactions Exist . The equilibrium method ...
Page 70
... usually sediment separately , thus avoiding the Johnston - Ogston effect . This will not necessarily be the case , however , if there are chemical reactions between the various species ; under these circumstances the 70 J. H. COATES.
... usually sediment separately , thus avoiding the Johnston - Ogston effect . This will not necessarily be the case , however , if there are chemical reactions between the various species ; under these circumstances the 70 J. H. COATES.
Page 71
... reactions have a nonzero value for their AV of reaction , the velocity of the zone will change with distance down the cell and with rotor speed because of the large pressure increase down the cell in zone sedimentation ( Kegeles et al ...
... reactions have a nonzero value for their AV of reaction , the velocity of the zone will change with distance down the cell and with rotor speed because of the large pressure increase down the cell in zone sedimentation ( Kegeles et al ...
Page 72
... reaction , the rate of movement of any reaction zone will be a function of both the position in the cell and the rotor speed . However , if either reactant is in excess and is completely resolved , it will move with its correct ...
... reaction , the rate of movement of any reaction zone will be a function of both the position in the cell and the rotor speed . However , if either reactant is in excess and is completely resolved , it will move with its correct ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero