Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 83
Page 19
... reference fringes for measurement purposes . Wedge window cells that deflect the emergent light beam about a radius through the center of rotation lying in the plane of the upper window are called left or right - side wedge windows ...
... reference fringes for measurement purposes . Wedge window cells that deflect the emergent light beam about a radius through the center of rotation lying in the plane of the upper window are called left or right - side wedge windows ...
Page 22
... reference edge ; 2 , outer reference edge ; 3 , air - solution meniscus ; 4 and 5 , inner and outer horizontal references generated by light passing through inner and outer reference holes . ( b ) Double sector cell , solution , and ...
... reference edge ; 2 , outer reference edge ; 3 , air - solution meniscus ; 4 and 5 , inner and outer horizontal references generated by light passing through inner and outer reference holes . ( b ) Double sector cell , solution , and ...
Page 24
... reference fringes ; 3 , reference wire image ( used to determine accurate x distances ) ; 4 , air fringes ; 5 , air - solution meniscus and air- solvent meniscus ; 6 , solution - oil meniscus . ( b ) 1 - mm column equilibrium patterns ...
... reference fringes ; 3 , reference wire image ( used to determine accurate x distances ) ; 4 , air fringes ; 5 , air - solution meniscus and air- solvent meniscus ; 6 , solution - oil meniscus . ( b ) 1 - mm column equilibrium patterns ...
Page 27
... reference edge ; 2 , air - solution meniscus ; 3 , boundary ; 4 , outer reference edge . low concentrations and in the case of an impure substance , that there is no interference by the other solute components . Two types of separation ...
... reference edge ; 2 , air - solution meniscus ; 3 , boundary ; 4 , outer reference edge . low concentrations and in the case of an impure substance , that there is no interference by the other solute components . Two types of separation ...
Page 29
... reference marks on the plate relative to the axes of the micro- scope stage . For measurements of sedimentation velocity schlieren plates , in which the position of the maximum ordinate along the horizontal axis only is required , only ...
... reference marks on the plate relative to the axes of the micro- scope stage . For measurements of sedimentation velocity schlieren plates , in which the position of the maximum ordinate along the horizontal axis only is required , only ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-ยน coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero