Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
From inside the book
Results 6-10 of 70
Page 21
... region is shown cross - hatched . The most deviated ray passes through the refractive index gradient maximum . 1 , Axis of rotation ; 2 , light source slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ; 6 ...
... region is shown cross - hatched . The most deviated ray passes through the refractive index gradient maximum . 1 , Axis of rotation ; 2 , light source slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ; 6 ...
Page 26
... region is shown cross - hatched . 1 , Axis of rotation ; 2 , light source slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ; 6 , camera lens ; 7 , photographic plate ; 8 , cell ; 9 , bromine - chlorine ...
... region is shown cross - hatched . 1 , Axis of rotation ; 2 , light source slit ; 3 , collimating lens ; 4 , condensing lens ; 5 , full surface 45 ° mirror ; 6 , camera lens ; 7 , photographic plate ; 8 , cell ; 9 , bromine - chlorine ...
Page 27
... region through the interface between the top and bottom halves . At the end of the run , the rotor is stopped and the total concentrations of solute in both top and bottom compartments determined . From the difference in concentration ...
... region through the interface between the top and bottom halves . At the end of the run , the rotor is stopped and the total concentrations of solute in both top and bottom compartments determined . From the difference in concentration ...
Page 28
... region can be carried out in such a system as can also zone sedimentation ( see Section VIII ) in which a band of solution less dense than the lightest solvent is placed on top of the solvent gradient . The sedimentation constants ...
... region can be carried out in such a system as can also zone sedimentation ( see Section VIII ) in which a band of solution less dense than the lightest solvent is placed on top of the solvent gradient . The sedimentation constants ...
Page 31
... region can be very helpful in aligning the imaginary line joining the reference fringes parallel to the x - axis of the microscope . The Rayleigh system essentially allows accurate measurement of differ- ences in refractive index ...
... region can be very helpful in aligning the imaginary line joining the reference fringes parallel to the x - axis of the microscope . The Rayleigh system essentially allows accurate measurement of differ- ences in refractive index ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero