Physical Principles and Techniques of Protein Chemistry Part C, Part 3Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry. |
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Page 31
... for the hydrolysis of simple phosphate esters as shown by Vandendriessche (1953).] More recent examples of studies on the time course of volume changes are those on the splitting of 17. DENSITY AND VOLUME MEASUREMENTS 31.
... for the hydrolysis of simple phosphate esters as shown by Vandendriessche (1953).] More recent examples of studies on the time course of volume changes are those on the splitting of 17. DENSITY AND VOLUME MEASUREMENTS 31.
Page 34
... studies of aqueous systems, the reference liquid is simply degassed pure water. Both the weights of the protein solution and the corresponding solvent medium are measured and related to the weight of water; in some cases the density of ...
... studies of aqueous systems, the reference liquid is simply degassed pure water. Both the weights of the protein solution and the corresponding solvent medium are measured and related to the weight of water; in some cases the density of ...
Page 36
... studies requiring many accurate density values or measurements which must be carried out quickly. Also, studies on the variation of pressure and temperature would pose severe difficulties. Nonetheless, this classical method is very ...
... studies requiring many accurate density values or measurements which must be carried out quickly. Also, studies on the variation of pressure and temperature would pose severe difficulties. Nonetheless, this classical method is very ...
Page 37
... studies as a function of temperature or pressure nor to those in which drastic difi'erences in density of the samples are encountered. Theoretical questions have been raised which may be diflicult to answer in a given case. Is the ...
... studies as a function of temperature or pressure nor to those in which drastic difi'erences in density of the samples are encountered. Theoretical questions have been raised which may be diflicult to answer in a given case. Is the ...
Page 48
... studies on a protein which was first made isoionic, if at all possible. From these considerations we might hazard an answer to the question posed at the outset. The investigator who wishes to know the correct value of v. for protein X ...
... studies on a protein which was first made isoionic, if at all possible. From these considerations we might hazard an answer to the question posed at the outset. The investigator who wishes to know the correct value of v. for protein X ...
Contents
1 | |
77 | |
Chapter 19 SmallAngle Xray Scattering | 141 |
Chapter 20 Pulsed Nuclear Magnetic Resonance | 245 |
Chapter 21 The Use of Least Squares in Data Analysis | 301 |
Chapter 22 Optical Rotatory Dispersion and the Main Chain Conformation of Proteins | 357 |
Chapter 23 Circular Dichroism | 445 |
Author Index | 595 |
Subject Index | 609 |
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Common terms and phrases
a-helix absorption acid Amer angle B-structure Beychok Biochem Biochemistry Biol Blout calculated CD band CD spectra Chem chromophore circular dichroism coefficient Cohn coil concentration conformation constant contribution Cotton effects creatine kinase cross-section defined definition denatured determined dialyzate diffusible components dipole disulfide electron density ellipticity enzyme equation equilibrium exciton experimental Fasman field find first fit function grams helix hemoglobin intensity interaction Kratky lysozyme magnetic measurements membrane method molal mole molecular weight molecule myoglobin negative negative band obtained optical activity ORD curve osmotic pressure parameters partial specific volume peptide Phys Pilz polymer polypeptides positive protein protein solution Pysh radius of gyration random coil residues ribonuclease rotational strength rotatory sample scattering curve Schellman Section shape significant small-angle solvent solvent medium spectrum studies subunits sufficiently temperature theoretical theory Tinoco tion transition tyrosine wavelength Woody X-ray zero