Physical Principles and Techniques of Protein Chemistry Part B, Part 2Sydney Leach Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry. |
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Results 6-10 of 88
Page 38
... values greater than 2 S. For lower values the transport method ( Section IV , B , 3 ) should be used . For s values up to about 20 S , maximum speed may be used . For more rapidly sedimenting species , the speed is reduced in proportion ...
... values greater than 2 S. For lower values the transport method ( Section IV , B , 3 ) should be used . For s values up to about 20 S , maximum speed may be used . For more rapidly sedimenting species , the speed is reduced in proportion ...
Page 39
... value calculated from the second moment is a weight average value ( Schachman , 1959 , p . 67 ) . With a schlieren ... values needed may be calculated from the X dis- tances at which the Y ; are measured , by means of Eq . ( 17 ) or Eq ...
... value calculated from the second moment is a weight average value ( Schachman , 1959 , p . 67 ) . With a schlieren ... values needed may be calculated from the X dis- tances at which the Y ; are measured , by means of Eq . ( 17 ) or Eq ...
Page 40
... values from comparator measurements using the maximum ordinate method ( Billick , 1962 ; Trautman , 1966 ; Moore , 1964 ) . The second - moment calculation . has been programmed for the computation of s values from raw com- parator ...
... values from comparator measurements using the maximum ordinate method ( Billick , 1962 ; Trautman , 1966 ; Moore , 1964 ) . The second - moment calculation . has been programmed for the computation of s values from raw com- parator ...
Page 41
... value . Values of s with a precision of ± 1 % can be obtained . This procedure gives a weight average value for s in the case of a polydisperse solute . b . Use of a Separation Cell . The cell ( Section II , D , 3 , a ) is divided into ...
... value . Values of s with a precision of ± 1 % can be obtained . This procedure gives a weight average value for s in the case of a polydisperse solute . b . Use of a Separation Cell . The cell ( Section II , D , 3 , a ) is divided into ...
Page 42
... values are used instead . is the partial specific volume of the protein and is assumed to remain constant . C. DETECTION OF HETEROGENEITY 1. Sedimentation Coefficient Distributions The appearance of two or more distinct peaks in a ...
... values are used instead . is the partial specific volume of the protein and is assumed to remain constant . C. DETECTION OF HETEROGENEITY 1. Sedimentation Coefficient Distributions The appearance of two or more distinct peaks in a ...
Contents
1 | |
Chapter 11 Viscosity | 99 |
Chapter 12 Light Scattering | 147 |
Chapter 13 Infrared Methods | 213 |
Chapter 14 Nuclear Magnetic Resonance Spectroscopy | 275 |
Chapter 15 Binding of Protons and Other Ions | 365 |
Chapter 16 Differential Thermal Analysis | 437 |
Author Index | 463 |
Subject Index | 479 |
Common terms and phrases
absorption anions atoms band beam binding Biochemistry Biol bond bound Bradbury calculated cell chain changes Chem chemical shifts cm-¹ coil complex component concentration conformational constant copper(II crystalline denaturation density gradient dependence determined differential thermal analysis effect electron enzyme equation equilibrium field Fraser frequency fringe Gurd histidine hydrogen ion imidazole imidazole groups instrument interaction intrinsic viscosity Jardetzky ligand light scattering light-scattering line width lysozyme macromolecule magnetic measured meniscus metal ion method molecular weight molecule myoglobin Natl nuclei observed obtained optical density orientation parameters partial specific volume particle peak peptide Phys Polymer Polymer Sci Proc protein solution protons random coil reaction reference refractive index region relaxation residues resonance RNase rotation rotor sample schlieren Section sedimentation coefficient slit solvent spectra spectrum speed structure studies Tanford technique temperature thermogram Timasheff tion titration transition transmittance ultracentrifuge values velocity Vinograd viscometer zero