Physical Principles and Techniques of Protein Chemistry Part B
Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then de
1 online resource (508 pages).
9780323141130, 0323141137
1044714497
Front Cover; Physical Principles and Techniques of Protein Chemistry, Part B ; Copyright Page; Table of Contents; List of Contributors; Preface; Contents of Other Parts; Chapter 10. Ultracentrifugal Analysis; Glossary of Symbols; I. Introduction; II. Fundamentals of the Method; III. Subsidiary Measurements Required in Ultracentrifugal Analysis; IV. Sedimentation Velocity Behavior of Noninteracting Solutes; V. Sedimentation Equilibrium of Noninteracting Solutes; VI. The Archibald Method. Equilibrium at the Menisci Only; VII. Sedimentation Behavior of Interacting Solutes. VIII. Zonal Analytical Velocity SedimentationIX. Isopycnic Density Gradient Sedimentation; X.A Brief Comparison of Some Commercially Available Ultracentrifuges; References; Chapter 11. Viscosity; Glossary of Symbols; I. Introduction; II. Viscosity and Intrinsic Viscosity; III. Experimental Methods; IV. Theories for ""Model"" Particles of Various Shapes; V. Dependence of Viscosity or Intrinsic Viscosity on Various Factors; VI. Use of Viscosity Measurements in Protein Chemistry; References; Chapter 12. Light Scattering; Glossary of Symbols; I. Introduction; II. Principles; III. Practice. AppendixReferences; Chapter 13. Infrared Methods; Glossary of Symbols; I. Introduction; II. Measurement of Spectra; III. Quantitative Analysis of Spectra; IV. Correlation of Spectra with Structure; References; Chapter 14. Nuclear Magnetic Resonance Spectroscopy; Glossary of Symbols; I. Introduction; II. Basic Theory of Nuclear Magnetic Resonance; III. Experimental Techniques; IV. Applications; References; Chapter 15. Binding of Protons and Other Ions; Glossary of Symbols; I. Introduction; II. General Considerations in Ion Binding. III. Specificity of Binding Environments Illustrated for Hydrogen IonsIV. Ion Binding in the Crystalline State; V. Ion Binding by Native Proteins in Solution; VI. Major Conformational Changes Related to Ion Binding; VII. Some Problems for Future Study; References; Chapter 16. Differential Thermal Analysis; I. Introduction; II. The Basic Principles; III. Presentation of Data; IV. Instrumentation and Methodology; V. Limitations; VI. Applications to Polymers; VII. Protein Analyses; VIII. Future Applications; IX. Complementary Methods; References; Author Index; Subject Index