Physical Principles and Techniques of Protein Chemistry Part A
Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of
1 online resource (545 pages).
9780323141123, 0323141129
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Front Cover; Physical Principles and Techniques of Protein Chemistry; Copyright Page; Table of Contents; List of Contributors; Preface; Contents of Part B; Chapter 1. Electron Microscopy of Globular Proteins; I. Introduction; II. Electron Microscopy and the Electron Microscope; III. The Enhancement of Contrast; IV. The Preservation of Specimens; V. Measurement; VI. Examples of the Application of Electron Microscopy to the Study of Proteins; VII. Prospects in the Electron Microscopy of Proteins; References; Chapter 2. X-Ray Methods; Glossary of Symbols; I. Introduction. II. The Nature of X-Ray DiffractionIII. The Protein Crystal; IV. The Reciprocal Lattice; V. The Structure Factor; VI. Fourier Synthesis; VII. Methods of Structure Determination; VIII. Fiber Diffraction; IX. Further Reading; References; Chapter 3. Ultraviolet Absorption; Glossary of Symbols; I. Introduction; II. Basic Spectroscopic Considerations; III. Chromophores of Proteins; IV. Spectrophotometric Titrations; V. Perturbation Difference Spectra; VI. Applications of Difference Spectra; VII. Determination of Tyrosine and Tryptophan; VIII. Experimental Considerations; IX. Prospects; References. Chapter 4. Fluorescence of ProteinsGlossary of Symbols; I. Theory; II. Technique; III. Application to Proteins and Model Compounds; References; Chapter 5. Perturbation and Flow Techniques; Glossary of Symbols; I. Introduction: On the Nature of Kinetic Analysis; II. Perturbation Methods; III. Flow Techniques; IV. Combined Flow- and Temperature-Jump Techniques; References; Chapter 6. Dielectric Properties of Proteins I. Dielectric Relaxation; Glossary of Symbols; I. Introduction; II. Dielectrics in a Static Field; III. Dielectrics in a Periodic Electric Field. IV. Measurement of Dielectric Constant and Dielectric LossV. Dielectric Relaxation of Proteins; VI. Dielectric Relaxation of Polyamino Acids; References; Chapter 7. Dielectric Properties of Proteins II. Electric Birefringence and Dichroism; Glossary of Symbols; I. Introduction; II. Outline of Theory; III. Experimental Methods; IV. Experimental Results; V. Concluding Remarks; References; Chapter 8. Electrophoresis; Glossary of Symbols; I. Introduction; II. Theory of Electromigration; III. Moving-Boundary Electrophoresis; IV. Zone Electrophoresis. Appendix A: Differential Equation of Forced Diffusion in the Case of ElectrophoresisAppendix B: Formulation of a Theory of Electrophoresis for Interactions between Macromolecules and Small Molecules; References; Chapter 9. Analytical Gel Filtration; Glossary of Symbols; I. Introduction; II. Theory of Gel Filtration; III. Experimental Aspects; IV. Estimation of Molecular Weight; V. Interpretation of Elution Profiles in Terms of Solute Composition; VI. Studies of Rapid Chemical Equilibria; VII. Concluding Remarks; References; Author Index; Subject Index